Effect Of Necrotizing Enteritis On EGC Secretion Factor In Broilers And Study On Resisting Disease Of Bifidobacterium

Objective:This study mainly studied the colitis of broilers caused by clostridium perfringens and the prevention of intestinal histological changes caused by clostridium perfringens with bifidobacterium.Type A and gas clostridium capsular infection model is set up,test is divided into control group and prevention of infection,using immunohistochemical method to detect three groups of enteric glial cells secrete factors expression of GFAP.NT-3 with ELISA method to detect IL-6 in three groups of intestinal IFN-?changes the expression of inflammatory cytokines.Methods:In this study,clostridium perfringens type A(ATCC13124)was selected as the pathogen.120 1-day-old broiler chickens were randomly divided into 3 groups.The experimental group 1 was the blank control group,and the basic diet was fed.Experimental group 2 was the infected group,fed a basal diet and 1ml of 1.0×10~8CFU/ml clostridium perfringens solution by irrigation.Experimental group 3 was the prophylaxis group,which was fed a basal diet,administered 1.0×10~8CFU/ml clostridium percapsulatum solution 1ml,fed 4%bifidobacterium powder(the number of live bacteria was no less than 10~8CFU/g)and selected 7.14.21d.5chickens were randomly selected from the blank group infection group and the prophylaxis group respectively,and the duodenal jejunal ileum was sampledA small segment of cecum was removed,the intestinal contents were fixed in 10%formaldehyde,and paraffin sections were made.The colitis caused by clostridium perfringens was detected by HE staining and the pathological changes of colitis caused by clostridium perfringens were prevented by bifidobacterium.Duodenal jejunum ileum cecum was immobilized with 4%paraformaldehyde and paraffin sections were prepared.The broilers'enteritis caused by clostridium perfringens was detected by immunohistohistotomy and bifidobacterium was used to prevent the expression of glial cell secreting factor GFAP NT-3 in broilers'enteritis caused by clostridium perfringens.Fresh intestinal tissue was collected,ground and centrifugated.ELISA was used to detect the expression changes of IL-6 IFN-?in broiler colitis caused by clostridium perfringens and bifidobacterium to prevent the expression changes of IL-6 IFN-?in broiler colitis caused by clostridium perfringens.Result:1.Intestinal pathological changes:in the blank group,the chicken intestinal villi were relatively complete without bleeding.In the infected group,the intestinal villi were not complete,and there was obvious bleeding in the lamina propria of the villi.The intestinal villi in the prevention group were relatively complete with normal structure.2.Expression of GFAP NT-3 secreted by enteral glial cells:in the blank group,GFAP was mainly strongly expressed in the submucosal intestinal gland of mucosomous myometrium of mucous epithelial lamina propria,and weakly expressed in the muscular layer,but not in the outer membrane.The infection group was mainly expressed in mucosal epithelium,mucous mucosa,muscularis mucosa,submucosal intestinal gland,but not in lamina propria,muscularis propria.Prevention group mainly in the muscularis propria mucosa submucosa intestinal mucosal epithelium strong expression,weak expression in the muscle layer,the outer membrane expression,positive expression score blank above prevention group,preventive group is higher than the infection in the NT-3 blank group mainly in the mucosa epithelium mucosa submucosa muscular intestinal strong expression,expressed in lamina propria muscular layer,outer membrane expression;In the infection group,the expression was strong in the mucosal submucosal intestinal glands of the mucosa and muscularis propria,but not in the muscularis.The prophylaxis group was mainly strongly expressed in the mucosal muscularis of mucous epithelium lamina propria,but not in the muscularis,and the total positive expression was higher in the infection group than in the prophylaxis group,and higher in the prophylaxis group than in the blank group.3.IL-6.IFN-?expression changes:poultry intestinal infection group IL-6 expression quantity higher than that of blank group prevention group,the extremely significant difference(P<0.01),the expression of blank group is higher than the amount of prevention group,no significant difference(P>0.05),almost at the same level of poultry intestinal infection group IFN-?expression quantity higher than that of blank group prevention group,significant difference(P<0.05),the expression of prevention amount higher than the blank group,no significant difference(P>0.05),almost at the same level.Conclusion:Clostridium perfringens caused chicken enteritis and the total expression score of intestinal glial cell secreted factor GFAP was lower than that of the blank group.Bifidobacterium had certain effect in preventing chicken enteritis caused by clostridium perfringens infection,which reduced the expression level of intestinal glial cell secreted factor GFAP and inhibited clostridium perfringens.The total expression score of the intestinal glial cell secreted factor nt-3 was higher than that of the blank group,which had a certain effect on the prevention of chicken enteritis caused by clostridium perfringens infection.It reduced the expression level of the intestinal glial cell secreted factor NT-3,and had an inhibitory effect on clostridium perfringens.Intestinal cytokine IL-6,IFN-?expression quantity is higher than the control group,bifidobacterium prevention caused by clostridium gas capsule infection chicken enteritis have certain effect,reduced intestinal cytokine IL-6 in the expression of IFN-?,significant difference,the clostridium capsule has inhibitory effect on product gas.