| Rape is the main oil crops in China,Plant height is an important plant type trait in achieving the goal of high-quality and high-yield breeding of rape,and it is also one of the factors that affect the lodging of rape.After lodging,rape has an important impact on yield and quality.Reducing the plant height properly can reduce the risk of lodging.The cultivation of dwarf resources is the key to dwarf breeding of rape.At present,there are few reports on the identified and identified dwarf stalk materials.There are not many available dwarf effective genes,and the molecular basis of rape height regulation is limited.As a result,the development of new dwarf varieties and large-scale promotion are slow.Brassica napus DW871 was upright in the whole growing period,The plant type is compact,completely resistant to lodging,and the node angle density is concentrated.The plant type is compact,completely resistant to lodging,and the pod density is concentrated,It is not the same type as the currently reported dwarf rape,large yield potential and is a low stem germplasm resource with domestic characteristics,which is conducive to mechanized cultivation.Therefore,it is of great significance to study the genetic basis of plant height genes and analyze the ecological adaptability to accelerate the dwarfing breeding process of rapeseed and further increase the yield of rapeseed.In this paper,the dwarf stalk germplasm resource DW871 was used as the material to study the effects of the same cultivation density and fertilization level on agronomic traits and economic traits in different environments.Defined initially the best ecological adaptation area of DW871.At the same time,using BSA-seq simplified genomic sequencing technology,located primarily the genes related to the height traits of DW871.The results are as follows:(1)Performance of DW871 agronomic traits in different pilotDW871 had significant differences in plant height,branch height,root neck diameter,primary effective branch,secondary effective branch and main inflorescence length among different pilots.The plant height of DW871 is the highest in the Sinan pilot(115.88cm),which is 26.72cm and42.11cm higher than the Huishui(89.16cm)and Huaxi(73.77cm)pilots respectively;Primary effective branch performed the highest in the Sinan pilot(16.26),6.04 and 4.47 higher than the Huishui(10.22)and Huaxi(11.79)pilots,respectively;The main inflorescence length of DW871 was the highest in the Sinan pilot(40.82cm),which was 5.46cm and 16.94cm higher than the Huishui(35.36cm)and Huaxi(23.88cm)pilots.(2)Performance of DW871 economic traits of different pilotsDW871 had significant differences in main inflorescence effective pod number,branch effective pod number,single plant effective pod number,thousand-kernel weight,single plant biological yield and yield among different pilots.The number of effective pods per plan of DW871was the highest in the Sinan pilot(588.46),which was 252.13 and 197.54more than Huishui(336.33)and Huaxi pilot(390.92);The yield of DW871 is the highest in the Sinan pilot,The average is 3715.02kg/hm~2;the Huaxi pilot is the second,the average yield is 3405.56kg/hm~2;the Huishui pilot has the lowest average yield,only 2338.00kg/hm~2,which is1376.22kg/hm~2 decrease compared with the Sinan pilot.The correlation analysis results show that the yield of DW871 is significantly positively correlated with daily average temperature,and extremely negatively correlated with rainfall,sunshine hours and relative humidity.It shows that the environment has greater impact on the yield of DW871.(3)Differences of DW871 quality among different pilotsThere were significant differences in oil content,protein,linoleic acid,linolenic acid and erucic acid among different pilots but no significant differences in glucosinolates.In Sinan pilot,DW871 had the highest oil content(45.59%)and glucosinolates(26.60?mol/g),the lowest protein content(26.41%),linolenic acid(5.78%)and erucic acid(1.39%);in Huishui pilot,protein content(29.26%)and linoleic acid content(14.31%)were the highest;in Huaxi pilot,linolenic acid content(6.97%)and erucic acid content(2.76%)were the highest.It showed that the seed quality of DW871 was better under the environment of higher temperature,more rainfall and more sunshine hours.The experiment shows that DW871 has better adaptability in Sinan pilot,and it is suitable to popularize production in higher environmental temperature,Long sunshine time,heavy rainfall,low relative humidity,less evaporation and similar ecological environment.In the production and planting,we should pay attention to the prevention and control of Sclerotinia and improve the field management measures.(4)Location of plant height gene of new dwarf line DW871 in rapeA total of 121998 SNPs were detected between 47 dwarves and 47high-stem mixed gene pools.1582 SNPs resulting in non-synonymous mutations were located on ChrA10 chromosome,Interval length is 6.39MB,including 1405 candidate genes.Out of 1752011 SNPs that were obtained between HR and HDR pools 27723 SNPs led to the non-synonymous mutations.A total of 19 candidate loci were mapped on ChrA03,ChrA04,ChrA06,ChrA07,ChrA10 and ChrC03,Interval length is5.35 Mb,including 1143 candidate genes.However,these locus showed un-significant associations and need to be further verified.The 47 dwarf gene pools and 10 pure dwarf gene pools detected at 12.87-14.70 Mb on the ChrA10 chromosome coincided with each other,and the length of the coincidence interval was 1.83 Mb.As a summary,the qualitative locus controlling the plant height in DW871 was mapped at 12.87-14.70 Mb correlation interval on ChrA10chromosome,The length of the coincidence interval was 1.83 Mb.This result laid a good foundation for the future fine mapping of the functional gene in Brassica napus L. |
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