| Admois.ramossa is a perennial wild herbaceous plant on the side of Admois.L in Ranunculaceae.It is concentrated in the mountains of the south-eastern province of Liaoning and Jilin.Currently,it has been barely developed and applied in a practical way,and also lacking of related studies as well.In recent years,the number of wild resources has declined dramatically due to the ongoing deterioration of the environment.The technology of sexual reproduction and asexual reproduction has not been established.This paper will introduce the available methods of cultivated A.ramossa.Also,its biological characteristics,reproductive characteristics of seeds and rapid spread technology in vitro will be identified via this research.Additionally,the observation of the entire life cycle of living habits,morphological features of different organs,floral phenology and floral bud differentiation will be involved in this paper.Then,through the examination of effects of seed soaking temperature,GA3 concentration and low-temperature wet sand storage period on seed germination,the technology of sexual reproduction and asexual reproduction has not been established the effects of disinfection time,different hormone types and concentrations on regeneration in vitro will be calculated.Finally,aimed to provide practical evidence for the cultivation management and industrial reproduction of A.ramossa.The findings are as follows:（1）The quantitative characteristics of individual plant components of A.ramossa in different development periods are phenotypic plasticity,and there is a significant（P<0.05）linear positive correlation between the propagation or storage components in each period.The numbers of flowering time in the year of cultivating A.ramossa in 2018 was one week later than that in 2019,and the period of flowering was shorter.The flowering process has displayed multiple "M" curve for two consecutive years.The A.ramossa is sensitive to sunlight and its petals are closed under the rainy weather.According to the analysis,the difference of flowering phenology is influenced by the different cultivation environment and the different climate in the year of introduction.The flower bud differentiation starts at 45 days after the aboveground part withered for a 4-month duration.It is 20 days earlier than A.amurensis Regal et Radde.In this analysis,the floral bud differentiation was divided into 6 cycles according to histomorphological observations: undifferentiated period,floral primordium differentiation period,calyx differentiation period,petal differentiation period,stamen differentiation period and pistil differentiation period.（2）The mature seeds of A.ramossa are yellow-green,with a thick net layer.Seed weight of 1000 grains is 7.83 g.The water absorption appears to saturate within 6 hours.The seeds are excellent at absorbing water.The consistency of the seeds improved after water absorption by 58.66%.The preliminary decision was that the low rate of germination of seeds is not due to the seed coat mechanical barrier.Physiological dormancy exists in seeds.There are three feasible approaches might break the seed dormancy,such as seed soaking in warm soup,treatment with GA3,and storage of low-temperature sand.After 10 days of wet sand storage at 4℃,taking the seed in warm soup for 25 minutes at 25℃,GA3 concentration is 250 mg·L-14℃ for 12 h is the optimal treatment and the germination rate is 83.6%.（3）The technique of rapid propagation of A.ramossa in vitro is not mature.In this study,the best explants for tissue culture and propagation were stem segments with axillary buds.The ideal culture-medium for adventitious buds is MS + NAA 0.3 mg·L-1 + TDZ 0.5 mg·L-1 + 6-BA 1.0 mg·L-1,with an induction rate of 85.49%.The addition of 100 mg·L-1 ascorbic acids（Vc）to the above medium can effectively inhibit the browning of explants.The appropriate culture-medium for adventitious proliferation of the bud is MS + NAA 0.1 mg·L-1 + TDZ 1.0 mg·L-1 + 6-BA 0.5 mg·L-1 and the coefficient of proliferation is 5.45 The ideal culture-medium of seedling rooting is MS + NAA 0.5 mg·L-1 + IBA 0.5 mg·L-1,with a rooting rate of 66.02%.Initially,an in vitro regeneration technology system of A.ramossa was developed. |
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