| Objective:To observe the protective effect of PNS,ginsenoside Rg1,Rb1 and Re from Panax notoginseng(Burk.)F.H.Chen on intestinal and lung injury induced by intestinal ischemia-reperfusion in rat model.Methods:Part I:A total of 50 male Sprague-Dawley rates were divided into control group(10 animals)and ?/R group(40 animals).The rats in control group were subjected to laparotomy alone,and the rats in II/R group were subjected to 1 hour of superior mesenteric artery occlusion,followed by 2-hour,4-hour,8-hour and 12-hour reperfusion,respectively.Then the rats in ?/R group was divided into four groups according to the four reperfusion time points(?/R 2h group,?/R 4h group,?/R 8h group,?/R 12h group).At the end of experiment,all animals were killed.Intestinal and lung samples were immediately dissected out and washed with ice cold saline to remove excessive blood.The intestinal and lung samples were divided into three parts for different use,with one immediately stored at 80 ? for future analysis,another one excised and fixed in 10%formalin solution for H&E staining,and the third one stoved and determined wet-dry weights ratio.Intestinal and lung MPO and MDA were assayed according to the instructions of the commercially available kits.Part II:A total of 80 male Sprague-Dawley rates were randomly divided into ten groups including sham group,II/R model group,high-and low-dosages of PNS-pretreated groups,ginsenoside Rg1-pretreated groups,ginsenoside Rb1-pretreated groups and ginsenoside Re-pretreated groups.The rats in high-and low-dosages of PNS-pretreated groups were orally administrated PNS 63mg·kg-1·d-1 and 31.5 mg·kg-1·d-1;the rats in high-and low-dosages of ginsenoside Rg1-or Rb1-pretreated groups were orally administrated ginsenoside Rg1 or Rb1 19 mg·kg-1·d-1 and 9.5 mg·kg-1·d-1,respectively;The rats in high-and low-dosages of ginsenoside Re-pretreated groups were orally administrated ginsenoside Re 1.6 mg kg-1·d-1 or 0.8 mg·kg-1·d-1;and the animals in the sham and model groups were given intragastric an equivalent normal saline.After a pretreatment for 5 days,the animals in the model or pretreated groups were subjected to 1 hour of superior mesenteric artery occlusion,followed by 2 hours reperfusion to induce intestinal and lung injury,and the rats in sham group were subjected to laparotomy alone.All animals were killed 3 hours after operation and serum samples were collected into tubes.Intestinal and lung samples were dissected out and washed to remove excessive blood.Then the intestinal and lung samples were divided into two parts,one for H&E staining and another for wet-dry weights ratio.The levels of serum MDA,SOD,IL-10,IL-6 and TNF-a were assayed according to the instructions of the commercially available kits.Results:Part I:After reperfusion 2 hours there were morphological changes in the intestine mucosa,and extending the time of reperfusion to 8 hours it became worse.In II/R 12hours group,the damage of intestine mucosa tended to recovery.The levels of MPO,MDA and W/D ratio were markedly higher in 2h and 4h reperfusion time groups than control group(P<0.01 or P<0.05).After extending the time of reperfusion,the injury of lung tissue became worse and the levels of MPO,MDA and W/D ratio were markedly higher in 2h and 4h reperfusion time groups than control group(P<0.01 or P<0.05).Part ?:(1)Pathological intestinal damages were much lighter in PNS,ginsenoside Rg1,Rb1 and Re groups than ?/R group,and W/D ratio were significantly lower in PNS,ginsenoside Rg1,Rb1 and Re groups than in II/R group(P<0.01 or P<0.05).(2)The levels of TNF-a and IL-6 were markedly lower in PNS,ginsenoside Rg1,Rb1 and Re groups than ?/R group(P<0.01 or P<0.05).Ratio of IL-6/IL-10 and TNF-?/IL-10 were decrease in PNS,ginsenoside Rg1,Rb1 and Re groups(P<0.01 or P<0.05).(3)The content of MDA was significantly decreased in PNS,ginsenoside Rg1,Rb1 and Re groups compared with ?/R group,and the activity of SOD was obviously increased in PNS,ginsenoside Rg1,Rb1 and Re groups(P<0.01).Conclusions:Panax notoginseng saponins,ginsenoside Rg1,Rb1 and Re from Panax notoginseng(Burk.)F.H.Chen protects against intestinal ischemia-reperfusion induced intestinal and lung injury in rats via scavenging free radicals,inhibiting lipid peroxidation damage and inhibiting in inflammatory response. |
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