Study The Effect Of Targeted Therapy Of Pannexin3 On Osteoarthritis With Cell Culture Experimental

Objective: Osteoarthritis is a common and frequently occurring disease.Although it has been extensively studied,the pathogenesis of osteoarthritis is still not well-established to date.The predominant view believes that metabolic balance disturbance in articular cartilage is the leading cause of osteoarthritis and Pannexin3 plays a crucial role in the synthesis and metabolism of articular cartilage.The present study was designed to determine whether inhibition of Pannexin3 is effective in the treatment of osteoarthritis in an in vitro cell model established.Methods: Articular cartilage tissue of patients undergoing total knee arthroplasty for osteoarthritis was collected and a cell model was established.Cells in the experimental group were transfected with siRNA and those in the control group were transfected with NC by lipofectamine.Expression levels of MMP-1 and MMP-13 mRNAs and proteins in cells were detected by RT-PCR and Western-blot,respectively.Data obtained were analyzed with independent-samples T test.Results: Following about 14 days(first generation)and 8 days(second generation)incubation,chondrocytes could adhere to 80%-90% of the bottom of the bottle.The first two generations were less differentiated and could be stained yellow brown by type II collagen using immunohistochemistry.After24 hours of transfection,fluorescence expression was observed in about 80%of the observed cells.The mRNA and protein levels of MMP-1 and MMP-13 in the experimental group were significantly decreased compared with that of the control group and the differences were statistically significant(P<0.05).Conclusion: Inhibition of Pannexin3 gene resulted in the decreased expression of MMP-1,MMP-13 mRNAs and proteins in osteoarthritis chondrocytes.Pannexin3 may serve as a potential therapeutic target for the treatment of osteoarthritis.