Expression And Correlation Analysis Of SREBP1、P-AKT And HER2 In Endometrial Carcinoma

Objective:To investigate the expressions,correlation and significance of SREBP1,P-AKT and HER2 in different types of endometrial carcinoma,and to provide a theoretical basis for further exploration of the pathogenesis of endometrial carcinoma.Method:Collected 77 cases of endometrial adenocarcinoma（EA）,20cases of uterine serous carcinoma（USC）;20 cases of proliferative phase of endometrium tissues（PE）;20 cases of edometrial hyperplasia without atypia（EH）;20 cases of typical endometrial hyperplasia（AEH）from2006-2016 in Pathology department of Affiliated Hospital of North Sichuan Medical College and Nanchong Central Hospital.The expression of SREBP1and P-AKT in these tissues were detected by immunohistochemistry Maxvision^TM method.In additional,we also detected the expression of HER2,P53 and Ki67 in EA and USC tissues.Analysis the biomarker expressions correlated with clinicopathologic variables and with patient survival time.Result:1 The positive rates of SREBP1 in PE,EH,AEH,EA and USC were 20%,10%,65%,51.9%and 85%,respectively.The expression rate in AEH,EA and USC tissues were significantly higher than that in PE and EH groups（P<0.05）.The positive expression rate of SREBP1 in USC group was significantly higher than that in EA group.The positive rates of P-AKT expression in PE,EH,AEH,EA and USC tissues were 25%,25%,80%,89.6%,80%,respectively,and the positive expression rate of P-AKT in AEH,EA and USC group were significantly higher than that in PE and USC groups（P<0.05）,but there was no significant difference in the expression of P-AKT between AEH,EA and USC groups.The expression of HER2,P53,Ki67 in USC group were significantly higher than those in EA group,the differences were statistically significant（P<0.05）.2 In endometrioid adenocarcinoma,there was a positive correlation between P53 and Ki67（RR=0.349,P=0.002）,but the correlation between any other two factors were not significant（P>0.05）.In uterine serous carcinoma,the expression of SREBP1 was positively correlated with the expression of P-AKT（RR=0.490,P=0.028）,but the correlation between any other two factors were not significant（P>0.05）.3 In endometrioid adenocarcinoma,the serum total cholesterol of SREBP1 positive group was higher than the SREBP1 negative group（P<0.05）.But there was no significant difference in total glyceride（TG）and Low density lipoprotein cholesterol（LDL-C）between the two groups.In EA and USC patients,there showed no significant difference in the expression of SREBP1 and P-AKT between different pathological grades,different FIGO stages,myometrial invasion,lymph node metastasis,menopause and whether the age was over 50 years old or not（P>0.05）.4 The 1,5 and 10 year survival rates of the EA group were100.0%,92.0%,62.0%,respectively,and the 1-and 3-year survival rates of USC group were respectively 71.43%and 33.33%.Kaplan-meier survival analysis showed that the survival time of patients with EA was significantly higher than that in patients with USC.In patients with EA,the prognosis with SREBP1 negative group was significantly higher than that of SREBP1positive group（P<0.05）,Ki67 negative group have better prognosis than patients with Ki67 positive group（P<0.05）.P-AKT,HER2 and P53 had no significant correlation with patients’prognosis.But in patients with USC,SREBP1,P-AKT,HER2,P53 and Ki67 were not significantly correlated with the prognosis of the patients.Multivariate analysis showed that histological type was an independent factor affecting the survival time of patients（RR=0.057,95%CI 0.005⁰.701,P=0.025）.Conclusion:1 SREBP1 and P-AKT were over expressioned in AEH,EA,USC tissues,but expressioned very low in PE and EH tissues.It was speculated that SREBP1 and P-AKT were both involved in the process of the development and progression of endometrial carcinoma,and might interrelated with the malignancy degree.Further study in their mechanism of action in EA and USC might find a common point of the pathogenesis of two types of endometrial cancer.2 In the patients with EA,the high expression of SREBP1 was related to the increase of TC level,but not significantly related to the level of serum TG and LDL-C.The relationship between the local high expression of SREBP1and the serum lipid components need further study.3 Both in EA and USC tissues,the expressions of SREBP1,P-AKT,HER2,P53 and Ki67 were not statistically different among different pathological grades,FIGO stages,myometrial invasion,lymph node metastasis,menopause,and whether the age was over 50 years old or not.4 In EA tissues,there was no significantly correlation between SREBP1,P-AKT and HER2.We speculated that there’re any other factors which involved in the HER2/P-AKT/SREBP1 pathway.However,the SREBP1expression presented a notable positive correlation with P-AKT in USC tissues.It showed that SREBP1 and P-AKT might work together in promoting the development and progression of USC.5 The expression of HER2,P53 and Ki67 were related to patients’survival time.In patients with EA,it was presumed that the expression of SREBP1 might be used as an indicator of its prognosis.But they were not significance related in USC patients.P-AKT was not significance related survival both in EA and USC patients.