| Objective: To tutor the national natural science fund project bowel disease party "chung wan,day pivot,giant as the imaginary" as the breakthrough point,to observe the fire needle intervention diarrhea diarrhea(functional)the effect of pixu(spleen deficient),further explore the influence of fire on immune mechanism.Methods:Will clean level 40 SD rats(male and female half),weighing,serial number,divided into two groups according to random number table(n = 10),model group(n = 10),electric acupuncture group(n = 10),electric acupuncture with fire needle group(n = 10).Animals in the clean animal room,the adaptive feeding 5 days record the rat weight,begin to make the mold.Normal group daily and afternoon physiological saline 2ml / 100g;Groups on a daily,afternoon to give 1 ml/g raw rhubarb decoction 2 ml / 100 g,put rats in daily afternoon swim in the water temperature of 20 to 22 ? water tank,for trying to degrees: rotation of rats and tip into the water;Eating from 8 a.m.to 5 p.m.,the rest of the time is fasting,drinking water,and making the mold for 14 days.After the success of the model,the mice were recorded on the weight of the rat,the normal group,the model group did not intervene,and the diet was normal.Electric acupuncture group,with sweat medical card acupuncture needle acupuncture points(day pivot,chung wan,giant virtual),once per day,every time 15 minutes,including frequency of 50 hz,it is beautiful body shaking slightly,dilatational wave;Electric acupuncture with fire needle group,1,6 days for fire needle intervention,only partial iodine fell after disinfection,selection of fine,to pinpoint the needle body burned to white speed thorn disease acupuncture points(day pivot,chung wan,giant virtual);In the 2nd,3rd,4th,5th and 7th days,only the electric needle,method and the electric needle group.Intervention in 7 days.After the last acupuncture treatment,the fasting 24 hours,weighing each group of rats,was given 1ml / 100g3 % D-xylose solution.1 hours later,the rat abdominal cavity anesthesia,stripping abdominal aorta take blood,detection of serum D-xylose,red blood cell receptors and erythrocyte immune complex lei lei rate rate,weighing and stripping of spleen and thymus.Results:(1)Electric acupuncture,cupping plus fire needle intervention after 7 days,electric acupuncture,cupping plus fire needle group big ShuFen gradually hardens,into a grain of grain,eat more,between the rat activity increased,pushing,extrados phenomenon is not obvious,hair get close;The uninterfered model group rats,with a small increase in the amount of food,and the gradual easing of diarrhoea,become a bunch or a grain.End of the building,model group,electric acupuncture,cupping plus fire needle group compared with normal group,the weight have significant difference,statistically significant(P < 0.01),but no difference between three groups;After the intervention,the weight of the model group,the electric needle group,the needle and the needle group was significantly different than the normal group,which was statistically significant(P < 0.01).Model group,electric acupuncture,cupping plus fire needle group three groups compared to each other,electric acupuncture,cupping plus fire needle weight than the model group rats have to speed up the growth trend,but through the digital processing have no significance(P > 0.05).;(2)the fire needle acupoints "bowel disease" can improve the function of small intestine absorption,improve pixu(spleen deficient)diarrhea diarrhea(functional)in the rat serum D-xylose content: model group compared with normal group,the serum D-xylose content have significant difference,statistically significant(P < 0.01);Electric acupuncture group,the electric acupuncture with fire needle group have significant difference compared with model group,with statistical significance(P < 0.01);Electric acupuncture group compared with normal group,there are significant differences,statistically significant(P < 0.05),electric acupuncture with fire needle group compared with normal group,has no statistical significance(P > 0.05);Electric acupuncture group compared with electric acupuncture with fire needle group,there was a significant difference,statistically significant(P < 0.05).(3)the fire "bowel disease" acupoints can improve pixu(spleen deficient)diarrhea diarrhea(functional)rats spleen and thymus index.The spleen index: model group compared with normal group,there was a significant difference,statistically significant(P < 0.01);Electric acupuncture group compared with model group,no difference(P > 0.05),electric acupuncture with fire needle group have significant difference compared with model group,with statistical significance(P < 0.01);Electric acupuncture group with no difference compared with normal group(P < 0.05),electric acupuncture with fire needle group no difference compared with normal group(P > 0.05);Electric acupuncture group compared with electric acupuncture with fire needle group,there was a significant difference,statistically significant(P < 0.05),thymus index: model group compared with normal group,there was a significant difference,statistically significant(P < 0.01);Electric acupuncture group compared with model group,no difference(P > 0.05),electric acupuncture with fire needle group have significant difference compared with model group,with statistical significance(P < 0.01);Electric acupuncture group with no difference compared with normal group(P < 0.01),electric acupuncture with fire needle group no difference compared with normal group(P > 0.05);Electric acupuncture group compared with electric acupuncture with fire needle group,there was a significant difference,statistically significant(P < 0.05).(4)the fire needle acupoints "bowel disease" can improve the deficiency diarrhea rate of rat red blood cell receptor wreath wreath rate and lower erythrocyte immune complex receptors.Changes in the rate of red blood cell receptor wreath: model group compared with normal group have significant difference,statistically significant(P < 0.01);Model group compared with electric acupuncture group has no statistical significance(P > 0.05),the model group compared with electric acupuncture with fire needle group have significant difference,statistically significant(P < 0.01);The curative group compared with normal group have significant differences,statistically significant(P < 0.01);Electric acupuncture compared with fire needle group compared with normal group had obvious difference(P < 0.05);The curative group compared with electric acupuncture with fire needle group have significant differences,statistically significant(P < 0.01).Red blood cell immune complex changes in the rate of the wreath: model group compared with normal group have significant differences,statistically significant(P < 0.01);Model group compared with electric acupuncture group has no statistical significance(P > 0.05),the model group compared with electric acupuncture with fire needle group have significant difference,statistically significant(P < 0.01);The curative group compared with normal group have significant differences,statistically significant(P < 0.05);Electric acupuncture with fire needle group no difference compared with normal group(P > 0.05);The curative group compared with electric acupuncture with fire needle group have significant differences,statistically significant(P < 0.05).Conclusion:Acupuncture has obvious anti-diarrhea effect;Acupuncture to improve intestinal absorption function is one of the important ways to acupuncture perlocutionary;Acupuncture to improve immune function may be the important mechanism of the acupuncture perlocutionary;Fire needle plus cupping both anti-diarrhea effect,and improve the function of small intestine absorption and immune function,is superior to pure filiform needle.Fire has obvious advantages,fire needle is recommended for clinical promotion. |
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