| Scrophulariiflora is a traditional Chinese medicine,is also commonly used in pediatrics medicine,medicine is one of the common clinical tutor Mr.Song Mingsuo conditioning children taste.In the tens of thousands of cases of pediatric clinical practice,mentor Mr.Song Mingsuo found a new function of the medicine,the medicine in order to better experience the resistance,function,to deepen tutor "on the treatment of children with febrile diseases" from the spleen and stomach theory understanding,which is a new function of the special drugs have more features of conventional medicine in the preliminary research.Objective:Objective to evaluate the therapeutic effect of Rhizoma Coptidis,and to discuss the specific components of Rhizoma Coptidis.Methods:1.Effects of different extracts of Rhizoma Coptidis: To observe the effects of different extraction huhuanglian purgation effect: 70 mice were randomly divided into 7 groups,10 rats in each group,according to the amount of 2g/kg,the dosage of each group were determined as follows: 1 group blank group was given distilled water,2 groups of rhubarb positive control(0.67g/kg),3 groups of decoction high dose(0.67g/kg)4,water decoction group(2g/kg),5 groups of picrorrhiza free Decoction low dose group(0.33g/kg),6 ethanol extract(0.64g/kg),7 groups of medicinal powder(2g/kg),intragastric volume according to pharmacopoeia 20ml/kg,was observed in 5h group The first time of diarrhea,stool stools weighing the total amount of calculation,loose stools rate,loose stool and diarrhea index;through experimental comparison of carbon propelling drugs to promote the rate of effect of small intestine of mice.Comparison of different solvent extracts from Rhizoma Picrorhizae purgative effect: 48 mice were randomly divided into 6 groups,namely control group(distilled water),petroleum ether group(0.0348g/kg),ethyl acetate,chloroform(0.308g/kg)group(0.154g/kg),n-butanol group(0.693g/kg),water group(1.694g/kg),the control group was given distilled water,other groups were treated with different solvent extracts from Rhizoma Picrorhizae.Intragastric volume was 0.5ml,with the observation index.2.Comparison of picrorhiza Different Decocting Time purgation effect: 40 mice were randomly divided into 4 groups,namely control group,picrorrhiza group according todifferent decocting time,dosage of 2g/kg,dosage of each group were determined as follows: 1 group blank group was given distilled water,2 groups of 20min(2g/kg),3groups of 40min(2g/kg),4 60min(2g/kg),intragastric volume was 20ml/kg,with the observed indexes.Experiment: 12 mice were randomly divided into 2 groups,respectively:blank control group(distilled water),vanillic acid(group 0.04g/kg),intragastric volume was 0.4ml,with the observation index.3.Comparison of different solvent extracts from Rhizoma Picrorhizae purgative effect:48 mice were randomly divided into 6 groups,namely control group(distilled water),petroleum ether group(0.0348g/kg),ethyl acetate,chloroform(0.308g/kg)group(0.154g/kg),n-butanol group(0.693g/kg),water group(1.694g/kg),the control group was given distilled water,other groups were treated with different solvent extracts from Rhizoma Picrorhizae.Intragastric volume was 20ml/kg,with the observation index.4.Analysis of picrorrhiza fingerprint and spectrum effect relationship: using HPLC-MS method,high performance liquid chromatographic fingerprint of Rhizoma Picrorhizae chromatography,and through HPLC-MS technology to identify the samples of picrorhiza fingerprint peaks in the home.And with the pharmacodynamic results through correlation analysis,clear produce active ingredient lapactic role.5.Experiment: 12 mice were randomly divided into 2 groups,respectively: blank control group(distilled water),vanillic acid(group 0.04g/kg),intragastric volume was20ml/kg,with the observation index.Results:1.Effects of different extracts of Rhizoma Coptidis:free Decoction high dose group compared with the control group in the stools,loose stools rate,degree of diarrhea,intestinal propulsive rate differences(P<0.05);rhubarb group compared with the control group in the stools,diarrhea rate,level,index and intestinal propulsive rate index there were difference(P<0.05);water decoction group compared to control group in the stools,diarrhea rate,level,significant differences between the index and the propelling rate of small intestine were found(P<0.01),and water decoction with free Decoction high dose group,rhubarb group on the intestinal propulsive rate respectively has the difference(P<0.05)and significant differences(P<0.01).2.Picrorhiza Different Decocting Time purgation effect: compared with the blankcontrol group 20 min group,40 min group,60 min group in the stools,diarrhea rate,level,index and intestinal propulsive rate and other indicators have significant differences(P<0.01);40min group compared with 20 min group differences in diarrhea index(P<0.05);60min group and 20 min group in the stools,there are differences on the intestinal propulsive rate(P < 0.05),there are significant differences in the comparison on the diarrhea index(P < 0.01).Two of the overall contrast test results can be obtained scrophulariiflora decoction had diarrhea effect,the There is a positive correlation between the effect and decocting time diarrhea.3.Scrophulariiflora different solvent extract purgative effect: ethyl acetate group and blank group in the stools,diarrhea rate,index index and intestinal propulsive rate,there were significant differences(P<0.01);ethyl acetate group and chloroform group in stools,rate,and in comparison,intestinal there were differences(P<0.05),there are significant differences in the diarrhea index(P<0.01).4.Analysis of picrorrhiza fingerprint and spectrum effect relationship:Respectively the fingerprint analysis of Rhizoma Picrorhizae control samples and samples by HPLC,found a total of 16 peaks,and according to the retention time and UV absorption spectrum for identification of known components,that 3# was the peak of vanillic acid,10# was the peak of Picroside II,14# was the peak of Picroside I.15# was the peak of Picroside III,16# was the peak of cinnamic acid were compared.Different picrorhiza extract and different solvent extracts of the total peak area and rate of intestinal propulsion of the correlation,the correlation coefficient R scrophulariiflora different solvent extracts were less than 0.9,the correlation is small;the correlation coefficient between the extract of Rhizoma Picrorhizae R > 0.9 There are 6 peaks,respectively 1#,3#,4#,5#,8#,16# peak,but only 3#,4#,Pearson,5# peak correlation analysis showed a difference(P < 0.05),3#,4#,5# peak on behalf of the composition has certain purgative activity is the main active substance effect of diarrhea and intestinal propulsion of picrorhiza in comparison to identify the known material,the vanillic acid has certain activity,and mainly exists in the decoction,the other two kinds of active components remains to be investigated.5.Experiment: vanillic acid group and blank group than in the stools,diarrhea,rate,index and intestinal propulsion rate were different(P<0.05).Conclusion:1.Research shows that Picrorhizae diarrhea and intestinal propulsioneffect and single medicine decoction had the best effect.2.the active ingredient of this function is to produce vanillic acid and other two kinds of unknown components. |
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