| Objective:The aims of current study are to explore the relationship of intestinal microbiota disturbance and metabolism and assist to evaluate the intestinal microbiota disorder after antibiotic usage.Materials and methods:The experimental group consisted of 23 children(10 males and 13 females)who were hospitalized in the Department of Respiratory,affiliated Children's Hospital of Suzhou University from May to September 2017,and these children all were diagnosed with community-acquired pneumonia and treated with antibiotics at the same time.They were 10 boys and 13 girls,aged from 1 to 3 years old,and average age is 1.66 years.The cases must meet the following criteria:(1)All of the children's age is under 3 years;(2)All of the children satisfied the diagnostic criteria for Community Acquired Pneumonia,and the pathogen is bacterium.(3)All of the children had a history of using antibiotics(such as Amoxicillin,cefmetazole,cefuroxime,cefdiazine,ceftriaxone,cefoperazone,azithromycin,etc.),and antibiotic usage time is from 3 to 7 days.(4)All of the children have not had infectious diseases including digestive and respiratory system infections,irritable bowel syndrome,Inflammatory bowel disease and other related diseases that may affect the homeostasis of intestinal microbiota.The control group was 16 children with normal physical examination in children's health section,including 8 boys and 8 girls and aged 1 to 4 years old,all meet the following criteria:(1)less than 3 years of age;(2)at least 4 weeks without history of digestive and respiratory system infections;(3)antibiotics are not used for at least 4 weeks;(4)excluding functional constipation,irritable bowel syndrome,functional dyspepsia and other gastrointestinal diseases.The concentration of short chain fatty acid(SCFA)in feces was detected by GC.And then the fresh fecal samples were inoculated into the betch fermentation systems,which contained Basal medium(YCFA),lactulose(Lau),raffinose(RAF),fructooligosaccharide(FOS),galactooligosaccharide(GOS),isomer(IMO),oligomeric mannitol(MOS),xylo oligosaccharide(XOS),inulin(INU),soluble starch(STA),mannitol(MAI),Xylitol(XYI)medium.The gas production and SCFA concentration at 24 h were detected.SPASS 19.0 was used to analyze the data.Results: 1.The contents of the total SCFA,propionic acid and butyric acid in the feces of infants in antibiotic group were significantly lower than those in control group(P < 0.05);but there is no difference in acetic acid value(P=0.65).2.The ratio of the percentage of acetic acid,propionic acid and butyric acid in normal control group was 59.1:20.9:15.6,and the ratio of the three acids in antibiotic group was 84.5:6.3:6.1.The contents of propionic acid and butyric acid in antibiotic group was significantly lower than that in normal control group(P < 0.05).3.After fermentation for 24 h,the contents of total SCFA,propionic acid and butyric acid in each oligosaccharide medium were significantly lower than those in the control group(P < 0.05).There was no difference in acetic acid contents in the media including YCFA,MAI,XYI;and the acetic acid content in the other mediums was lower than that in the control group(P < 0.05).4.The production of gas in these mediums(including FOS?GOS?IMO?MOS?INU?STA?MAI?XYI)in antibiotic group was significantly lower than that in the control group(P < 0.05).But there was no significant difference between the two groups in 24 h gas production(P > 0.05)in these mediums including LAU,RAU,XOS.Conclusion: 1.It is susggested that antibiotics have significant influence on intestinal microbiota to measure the total contents of SCFA,propionic acid and butyric acid in feces.2.Antibiotics can make the intestinal microbiota metabolism disturbed,and it mainly expresses that the ratio of the percentage of acetic acid,propionic acid and butyric acid lost balance.3.Antibiotics can inhibit or kill the normal intestinal microbiota,and it can also significantly inhibit the metabolic ability of the intestinal microbiota,which mainly includes the ability to produce the contents of SCFA,propionic acid and butyric acid;and some acetic acid-producing bacteria is also inhibited.4.Antibiotics can inhibit or kill the normal intestinal microbiota,and it can also significantly inhibit the ability to produce gas by intestinal microbiota fermentation. |
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