| Objective:The aims of current study are to explore metabolic changes of intestinal microflora after treating infants' community-acquired pneumonia with antibiotics under the influence of probiotics,and to further verify the effect of probiotics on relieving and recovering intestinal flora dysbiosis caused by the antibiotics.Materials and methods:The cases of this study were consisted of 30 children,who were hospitalized in the Department of Respiratory,affiliated Children's Hospital of Soochow University from August 2018 to November 2018.All the children were diagnosed with community-acquired pneumonia and treated with antibiotics.They were seventeen boys and thirteen girls,and their ages were from 1 to 3 years old,with an average age of 2.16years.The duration of antibiotics treatment was from 6 to 9 days,with an average duration of 7.40 days.The cases must meet the following criteria:(1)all the children satisfied the diagnostic criteria for Community Acquired Pneumonia.(2)all the children had been infected with bacteria.(3)all the children used antibiotics via vein.(4)all the children were free from infectious diseases other than respiratory system infections.And they didn't have inflammatory bowel disease,functional gastrointestinal disorders and other related diseases that may have an influence on intestinal microflora.These children were divided into probiotics group and control group.Fifteen of them were in the probiotics group,who received two pills of Clostridium butyricum combined with Bifidobacterium infants probiotics mixture twice a day for fourteen days during the antibiotics treatment.The other 15 of them were in the control group,who didn't receive probiotics during and after the antibiotics treatment.The times of fecal samples collection were on the 1st.7th and 21st days after the beginning of antibiotics treatment.After collecting these fecal samples,they were placed in basic medium(YCFA),lactulose(LAU),fructooligosaccharide(FOS),galactooligosaccharide(GOS),inulin(INU),soluble starch(STA),mannitol(MAI),and cultured in thermostat for 24 hours.The contents of short chain fatty acid(SCFA)in feces and in the various mediums were detected by gas chromatography.The gas production in the various mediums at 24h was detected.SPSS 22.0 was used to analyze the data.Results:1.The contents of total SCFA,acetic acid,propionic acid and butyric acid in feces of the probiotics group were significantly higher than those of the control group on the 7th and 2lst days after the beginning of antibiotics treatment(P<0.05).2.The ratios of the percentage of acetic acid,propionic acid and butyric acid in the probiotics group respectively were 68:18:9?63:22:11 on the 7th and 21st days after the beginning of antibiotics treatment.The ratios of the percentage of acetic acid,propionic acid and butyric acid in the control group respectively were 73:18:3>71:20:5 on the 7th and 21st days after the beginning of antibiotics treatment.And the ratios of the percentage of butyric acid in the two groups were significantly different(P<0.05).3.After the feces fermented in virto,the contents of total SCFA in the probiotics group(except for YCFA on the 21st day)were significantly higher than those of the control group on the 7th and 21st days after the beginning of antibiotics treatment(P<0.05).4.After the feces fermented in virto,the contents of acetic acid in the probiotics group(except for YCFA on the 21st day)were significantly higher than those of the control group on the 7th and 21st days after the beginning of antibiotics treatment(P<0.05).5.After the feces fermented in virto,the contents of propionic acid in the probiotics group(except for FOS on the 7th day)were significantly higher than those of the control group on the 7th and 21st days after the beginning of antibiotics treatment(P<0.05).6.After the feces fermented in virto,the contents of butyric acid in the probiotics group(except for INU)were significantly higher than those of the control group on the 7th and 21st days after the beginning of antibiotics treatment(P<0.05).Conclusion:1.After treating community-acquired pneumonia in infants with antibiotics,the contents of total SCFA,acetic acid,propionic acid and butyric acid by the metabolism of intestinal microflora decreased significantly.2.On the 21th day after the beginning of antibiotics treatment,the metabolic ability of the intestinal microflora is still lower.3.In the feces and fermented feces,the contents of total SCFA,acetic acid,propionic acid and butyric acid in the probiotics group were significantly higher than those in the control group on the 7th and 21st days after treating community-acquired pneumonia in infants with antibiotics under the influence of probiotics(Clostridium butyricum combined with Bifidobacterium infants probiotic mixture).From the view of metabolism of intestinal microflora,probiotics are effective in restoring intestinal flora dysbiosis caused by antibiotics.4.Determining the contents of short chain fatty acid in the feces and fermented feces between the probiotics group and the control group,it is confirmed that probiotics could improve the intestinal flora dysbiosis caused by antibiotics,promote the recovery of intestinal microflora after the use of antibiotics.5.The vitro fermentation model of intestinal microflora may be helpful to evaluate intestinal flora dysbiosis caused by antibiotics and provide a new method for evaluating the role of probiotics. |
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