| BACKROUND: In the worldwide,acute myocardial infarction(AMI)is one of the clinic types of coronary artery disease(CAD),which has high fatality rate.In recent years,the incidence of AMI is increasing in China year by year,although with a mass of chest pain center were established in the nationwide,AMI is still one of the most dangerous diseases to human health.With the rapid development of gene sequencing technology and which applications is more and more extensive,SNPs is becoming a hot spots in the field of medical research.Genome-wide association studies(GWAS)has been found that some SNPs in human chromosome 9 p21.3 can increase the risks of CAD,myocardial infarction(MI),and type 2 diabetes.The association of rs1333040 SNPs within the cyclin-dependent kinase inhibitor 2B antisense RNA1(CDKN2B-AS1)gene with AMI has been verified in Caucasian.However,little was known about this correlation between rs1333040 SNPs and AMI in the Chinese Han population,especially in Guangxi Province Han population.Therefore,the understanding of the rs1333040 SNPs with susceptibility,risk factors,clinical features and gene-environment interaction for being contributed to know the genetic characteristics and providing a new way to screen high-risk population,prevention and translational medical research for AMI.OBJECTIVES: To explore the relationship between CDKN2B-AS1 gene rs1333040 SNPs and AMI susceptibility,risk factors,clinical characteristics and some environmental factors in the Chinese Han population of Guangxi Province,as well as the influence of rs1333040 SNPs and environment interaction.METHODS: In this study,the blood samples of 334 AMI patients(AMI group)and 334 healthy people(control group)were collected in Chinese Han population of Guangxi Province,by extracting DNA,polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)and agarose gel electrophoresis to determine CDKN2B-AS1 gene rs1333040 genotypes.The AMI group was divided into different subgroups by different clinical features.Comparison of the frequencies of genotype and allele in two groups and AMI various subgroups to explore the relationship between rs1333040 and susceptibility,clinical characteristics(diagnosis time,different infarction location,serious complications and typical symptoms)for AMI,respectively.Non-conditional binary logistic regression analysis was used to evaluate the relationship of risk factors and gene-environment interaction for AMI.RESULTS: Comparison of generalized characteristics between two group,the mean age,BMI,the numbers(percentages)of subjects with cigarette and alcohol consumption,the levels of TC,TG,and LDL-C were higher in the AMI group than in the normal group(P < 0.05 for each).However,the serum HDL-C level was lower in the AMI group than in the control group(P < 0.001).The TT genotype and T allele frequencies were higher in AMI group than in the control group(58.08% and 76.05% vs.41.92% and 66.02%,p < 0.001 for each),respectively.Non-conditional binary logistic regression analysis showed that diabetes,high blood pressure,smoking,age,and TC were strongly associated with AMI risk,with OR values of 66.165,12.371,3.012,2.080,and 3.297,respectively.In contrast,HDL-C and rs1333040 CC genotype were negatively correlated with AMI risk,with OR values of 0.029 and 0.524(P < 0.001 for each).However,no significant differences were seen between the AMI group and control group in terms of correlation of BMI,sex,TG,alcohol consumption,and LDL-C with AMI risk(P > 0.05 for each).The frequency of the C allele of rs1333040 in patients with DT > 12 h was significantly lower than in other diagnosis time subgroups(P < 0.05 for each).There were no significant differences in the genotype and allele of rs1333040 between the controls group and the AMI subgroups(typical symptoms,serious complications,and infarction location,P > 0.05 for each).The subjects who and smoked ? 20 cigarettes/day had the TT genotype and carrying the minor C allele were at a 600.8% and 525.0% increased risk for AMI(P < 0.001),respectively.In addition,no interaction was seen between the presence of the minor C allele and BMI or alcohol consumption(P > 0.017 for each).CONCLUSIONS: The TT genotype of rs1333040 was positively correlated with AMI and increased the risk of AMI.In addition,we discovered that the C allele of rs1333040 was significantly correlated with AMI DT ? 12 hours,it may be contributed to early diagnosis of AMI.Also,the interaction between TT genotype and the minor C allele of rs1333040 and smoking appears to increase the risk of AMI. |
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