| Objective:Schizophrenia is a severe mental illness characterized by heterogeneity and diverse symptoms. The pathogenesis of schizophrenia is unclear. This disease has been proved to be related with complex interactions between genetic susceptibility and environmental insults. The aim of this study is to investgate the association between microRNA137 gene polymorphism,childhood trauma with schizophrenia.The study includes more environmental risk factors to better understanding the onset of schizophreniaMethods:This study included 195 cases accorded with the DSM - ? diagnostic criteria of stability and 183 healthy controls matched gender and age. The genotypes of rsl 625579 site located miR - 137 genes were detected by mass spectrometry method. We collected all participants' general demographic information, perinatal environmental risk factors, and assessed all the subjects of childhood trauma experience. The difference of genotypes and allele frequencies of single nucletide polymorphisms rsl625579 in miR-137 gene ,the difference of childhood trauma experience between patients and healthy controls,Identifying environment factors associated with the onset age in patients and the G×E were analyzed by SPSS 21.0 software. At last,establishing logisitic regression model covering genotypes,prenatal malnutrition, infection during pregnancy, family history and CTQ understanded pathogenesis of schizophrenia.Results:1. Genetic research results: duing to the low G allele frequency, combining GG and GT group compared with TT, genotypes of GG + GT and TT exhibited no significant differences between patients and controls (x 2 = 2.520, P = 2.520).Kaplan Meier -analysis of genotype and and age of onest difference (GG + GT = 19.91 ±4.6 and TT?21.57 ± 7.54 years),performaning the Log Rank test (P = 0.182).2. Childhood trauma experience in patients were comparised with the control group: the scores of physical abuse (PA), body ignore (PN), emotional abuse(EA), emotional neglect (EN), sexual abuse, poor family environment (FA) and childhood trauma score (CTQ) in patient groups were significantly higher than control group (P < 0.001).3. Logistic regression analysis model was adopted to analyze the biological and social factors between the early-onset schizophrenia(?18 years old) and the late-onset schizophrenia (> 18 years old), The study found the early-onset schizophrenia patients experienced more maternal malnutrition during pregnancy (OR = 2.43, P ?2.43), and the eldly-onset schizophrenia patients experienced maternal infection during pregnancy (OR = 0.23, P = 0.23),while the gender(P=0.75) * family history(P=0.90),genotype (P=0.08) had no statistical signi:ficance .4. No interaction of rs1625579 loci in miR137 gene and childhood trauma was found to affect schizophrenia. (p>0.05 )5. Logistic regression analysis model showed significant difference in family history(OR = 5.05, P = 0.002), maternal malnutrition during pregnancy (OR = 2, 22, P ?0.03), maternal infection during pregnancy (OR = 3.91, P = 3.91) and CTQ (OR =1.09, P < 0.01). But genotypes had no statistical significance (P > 0.05).Conclusion:1. Rs1625579 polymorphisms in miR-137 gene may not be associated with schizophrenia.2. Rs1625579 polymorphisms in miR-137 gene had no significant differences between genotype and and age of onest.3. The patiens had higher scores in Physical abuse, body ignore, emotional abuse,neglect abuse, sexual abuse and bad family environment.4. No interaction of rsl 625579 loci in miRl37 gene and childhood trauma was found to affect schizophrenia.5. The patients who experienced maternal malnutrition during pregnancy had an earlier age at onset. The patients who experienced maternal infection during pregnancy had an elder age at onset. The family history, maternal malnutrition during pregnancy, infection during pregnancy, childhood trauma all may be etiologically contribute to schizophrenia, but the mechanisms needs further research. |
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