Study On The Transcriptomsequencing And Pedigree Of CD133 Positive Neural Stem Cells From Central Spinal Canal In C57 Mice

Spinal cord injury(SCI,spinal cord injury)is a kind of nervous system damage,usually occured in movement,transportation,medical and engineering at all.The disease often leads to a series of serious consequences,,for example,Under injury,patients will lose some or all of function,weaken the bladder,bowel and sexual function,can also lead to extensor and flexor muscle spasms,making patients suffering great pain,let originally happy family at risk.The pathological feature of spinal cord injury is nervous tissue necrosis,neuronal apoptosis,at present the main treatment of surgery and drug therapy,however,these two methods are difficult to produce new neurons to replace the damaged neurons,the treatment effect is not ideal.In recent years,people have tried transplanted neural stem cells to treat spinal cord injury,some research progress has been made,but the transplant exogenous nerve cells often appear immune rejection,with the risk of tumor formation,but also involves ethical issues,mobilization of endogenous neural stem cells to repair spinal cord injury has a more broad application prospects.CD133 positive nerve stem cells exist in the ependymal area of the central spinal canal.Due to the low number and heterogeneity of neural stem cells,it is not possible to conduct expression spectrum analysis of these cells by conventional methods.CD133+ cells in the central canal of spinal cord of mice were studied by single cell transcriptome sequencing,First,a sufficient number of CD133 positive single cells and a certain number of CD133 negative cells were obtained from the central canal of mouse spinal cord through digestion and flow separation.Secondly,through analysis,we found that the characteristics of some cells in the central canal of spinal cord in mice were consistent with those of neural stem cells,which indicated that there were indeed neural stem cells in the central canal of spinal cord in mice.Thirdly,through bioinformatics analysis,we classified all the single cell samples and obtained four cell types,including stem cells,glial cells and neurons,This means that nerve stem cells in the central canal of the spinal cord can differentiate into glial cells and neurons.Fourth,we further analyzed the four cell types and obtained the relationship between the degree of differentiation of each cell type.Fifth,through the functional analysis of WGCNA gene,we found that stem cells may have the function of regulating cell proliferation,differentiation and development;Glial cells may have metabolic and electronic transport functions and are closely linked to diseases such as huntington's disease,Parkinson's disease and alzheimer's disease;neurons may regulate neuron development,neuron differentiation,and cell morphology.Our results provide a more detailed understanding of the cell types and differentiation lineages in the central canal of the spinal cord.Because endogenous neural stem cells can be used to repair the damage of the nervous system,This study has important reference value for the research of spinal cord injury diseases..