To Investigate The Immune Reconstruct Regularity Profile Of KIR Gene In Allo-HSCT

?Objectives?To investigate the immune reconstruct regularity profile of KIR 2DL1 and KIR3DL1 in allogeneic hematopoietic stem cell transplantation(allo-HSCT)with unrelated-donor with KIR-AA genotype.To investigate the immune reconstruct regularity profile of KIR 2DS1 and KIR 3DS1 in allogeneic hematopoietic stem cell transplantation(allo-HSCT)with unrelated-donor with KIR-Bx genotype.?Method?119 donor-recipient pairs were performed by KIR genotying using PCR-SSP,75-case donors were identified with KIR-AA genotype.Dynamic detection the expression of KIR2DL1/3DL1 on NK cell and m RNA level in 291 cases,included unrelated-donor on the day of transplantation and the recipient each month post transplantation,included,using flow cytometry(FCM)and real-time fluorescent quantitation PCR(RT-q PCR)methods.44-case donors were identified with KIR-Bx genotype.Dynamic detection the expression of KIR 2DS1/3DS1 on m RNA level in 196 cases,included unrelated-donor on the day of transplantation and the recipient each month post transplantation,using real-time fluorescent quantitation PCR methods.?Result?1.The Median expression of KIR 2DL1 in unrelated-donor on transplant's day was 21.60%,the median expression of KIR 2DL1 in recipient 1M,2M,3M and 3-6M after transplatation were 7.40%,12.0%,16.92%,17.64%.Median expression of KIR 2DL1 in unrelated-donor on transplant's day was 265.14 copies/10000 abl copies,the medianexpression of KIR 2DL1 in recipient 1M,2M,3M,3-6M,6-9M,9-12 M after transplatation were 332.17 copies,438.31 copies,723.25 copies,414.17 copies,180.76 copies,234.67copies/10000 abl copies.The median expression of KIR 2DL1 on NK cells and m RNA level are gradually increased at all time points after transplantation.But m RNA expression levels were increased earlier than NK cell membrane proteins.2.Median expression of KIR 3DL1 in unrelated-donors on transplant's day was18.56%,the median expression of KIR 3DL1 in recipient 1M,2M,3M,3-6M after transplatation were 23.83%,22.57%,23.02%,21.6%.Median expression of KIR 3DL1 in unrelated-donor on transplant's day was 572.29 copies/10000 abl copies,the median expression of KIR3DL1 in recipient 1M,2M,3M,3-6M,6-9M,9-12 M after transplatation were 1 233.74 copies,1 140.42 copies,876.73 copies,1 057.07 copies,739.02 copies,514.43 copies/10000 abl copies.The median expression of KIR 3DL1 on NK cells and m RNA level were higher than donors at 1 month after transplantation,and stable expression at all time points after transplantation,so m RNA and NK cell membrane proteins expression were increased at the same time.3.Median expression of KIR 2DS1 in unrelated-donor on transplant's day was1898.79 copies/10000 abl copies,the median expression of KIR 2DS1 in recipient 1M,2M,3M,3-6M,6-9M,9-12 M after transplatation were 2 576.56 copies,2 654.32 copies,2394.46 copies,1638.42 copies,1812.42 copies,2076.91copies/10000 ablcopies.Media n expression of KIR 3DS1 in unrelated-donor on transplant's day was 2479.92copies/10000 abl copies,the median expression of KIR 3DS1 in recipient 1M,2M,3M,3-6M,6-9M,9-12 M after transplatation were 3612.00 copies,5136.43 copies,6505.61 copies,5881.28 copies,5121.80 copies,5094.28 copies/10000 abl copies.4.Immune reconstitution of KIR 2DL1/2DS1 and KIR 3DL1/3DS1: The RNA expression of KIR2DL1 reach the highest at 3 month and the m RNA expression of 2DS1 peak the highest in the first month after transplantation.The m RNA expression of KIR3DL1 was highest at the first month and the expression of 3DS1 was highest at the third month after transplantation.Therefore,the immune reconstruct regularity of differentgenotypes of the inhibitory KIR gene and the activated KIR gene is different,that is to say,KIR 3DL1 is earlier than 2DL1,KIR 2DS1 is earlier than 2DL1,and KIR 3DL1 is earlier than 3DS1.?Conclusion?The immune reconstruct regularity of KIR 2DL1/2DS1 and KIR 3DL1/3DS1 gene are different,so it provided a theoretical basis for quantitative analyse the KIR gene expression at the right time and predict the prognosis of recipient after transplatation.At the same time,it provided an experimental basis for analyse the immune reconstruction of other i KIR and a KIR genes.