The Effect Mechanism Of MiR-21 On Invasion,Metastasis And Epithelialmesenchymal Transition Of Bladder Cancer T24 Clls

Objective: To investigate the effects of miR-21 on the invasion,metastasis and epithelial mesenchymal transition(EMT)in human bladder cancer cell.Methods: MiR-21 mimics,miR-21 inhibitors and the respective control were transfected into bladder cancer cell T24 by liposomes transfection method,the respective control was taken as the negative control group,the non-transfection group taken as blank control group.After 48 h,the effectiveness of transfection was observed by under fluorescence microscope.Cell scratch experiment and Transwell invasion experiment were used to detect the migration and invasion.Western blot was used to observe the expression of epithelial protein and mesenchymal protein of transfected T24 cells.Results: 1.The transfection efficiency of T24 cells observed under fluorescence microscope was about 75%.2.As revealed by Transwell invasion test of T24 cells: Compared with other groups,the number of invaded cells in miR-21 mimics transfection group were significantly higher,;the number of invaded cells in miR-21 inhibitors transfection group were significantly lower,(P<0.05);3.As revealed by the cell Scratch test of T24 cells: at 48 h after scratch,Compared with other groups,the healing rate of miR-21 mimics transfection group was significantly higher;the healing rate of miR-21 inhibitors transfection group were significantly lower,(P<0.05);4.As revealed by Western blot test of T24 cells: the protein levels of Ecadherin in mimic group was significantly down-regulated(p < 0.05).Contrast,Vimentin ?Slug were significantly up-regulated(p<0.05).Conclusion: MiR-21 overexpression could promot invasion,metastasis and EMT of human bladder cancer cells.