2-deoxy-D-glucose Reverses The Antagonism Role Of Hydrogen Sulfide In Hippocampal Synaptic Plasticity Of Postoperative Aged Rats

[Backgroud and Objective]Hydrogen sulfide?H₂S?,a new neuromodulator,improves hippocampal synaptic plasticity of postoperative aged rats.However,the specific mechanism is unclear.Warburg effect,known as aerobic glycolysis,plays an important regulatory role in the synaptic plasticity of hippocampus.Our previous work found H₂S upregulates hippocampcal Warburg effect.To further validate whether hippocampcal Warburg effect mediates the improving role of H₂S in hippocampal synaptic plasticity of postoperative aged rats,our present work explored whether2-DG,an inhibitor of Warburg effect,reverses the improving effect of H₂S on the hippocampal synaptic plasticity in the postoperative aged rats.[Methods]The expressions of hippocampal synaptic plasticity-associated proteins,including Synapsin-1 and PSD-95,were detected by immunohistochemistry and western blotting.Synaptic density and ultrastructure in the CA1 and CA3 regions of hippocampus were observed under transmission electron microscopy.[Results]1.2-DG reversed H₂S-upregulated hippocampal Synapsin-1expression in postoperative aged rats Immunohistochemistry and western blotting showed that NaHS?100?mol/kg/d,i.p.,7 d?significantly upregulated the expression of hippocampal Synapsin-1 in the postoperative aged rats.However,cotreatment with 2-DG obviously reversed NaHS-induced upregulation in the expression of hippocampal Synapsin-1 in the postoperative aged rats.2.2-DG reversed H₂S-upregulated hippocampal PSD-95 expression in postoperative aged rats Immunohistochemistry and western blotting showed that NaHS?100?mol/kg/d,i.p.,7 d?significantly upregulated the expression of hippocampal PSD-95 in the postoperative aged rats.However,cotreatment with 2-DG obviously reversed NaHS-induced upregulation in the expressions of hippocampal PSD-95 in the postoperative aged rats.3.2-DG reversed H₂S-increased hippocampal synaptic density in postoperative aged rats Transmission electron microscopy showed that NaHS?100?mol/kg,i.p.,7 d?significantly increased hippocampal synaptic density.However,cotreatment with 2-DG obviously reversed NaHS-induced increase in synaptic density of the hippocampal CA1 and CA3 regions in postoperative aged rats.4.2-DG reversed H₂S-improved hippocampal synaptic ultrastructure in postoperative aged rats Transmission electron microscopy showed that NaHS?100?mol/kg,i.p.,7 d?significantly increased the length of synaptic active zone and the thickness of postsynaptic density as well as reduced the width of synaptic gap in the hippocampal CA1 and CA3 regions of postoperative aged rats.However,cotreatment with 2-DG obviously reversed Na HS-induced increase in the length of synaptic active zone and the thickness of postsynaptic density as well as decrease in the width of synaptic gap in the hippocampal CA1 and CA3 regions of postoperative aged rats,which indicated that 2-DG reversed H₂S-improved hippocampal synaptic ultrastructure in postoperative aged rats.[Conclusion]2-DG reverses the improving effect of H₂S on the hippocampal synaptic plasticity of postoperative aged rats.