Hydrogen Sulfide Upregulated Warburg Effect In The Brain Of Postoprative Aged Rats And The Effect Of 2-deoxy-d-glucose

[Background and Objective]Hydrogen sulfide H₂S),as a novel neuro-protectant,ameliorates postoperative cognitive dysfunction POCD).However,the mechanisms remain largely unknown.Warburg effect aerobic glycolysis)plays an important role in regulating learning and memory.2-Deoxy-D-glucose 2-DG)is a recognized inhibitor of Warburg effect.The present work explored the influences of H₂ S on Warburg effect in the brain of postoperative aged rats and whether 2-DG inhibits H₂S-exerted change in the warburg effect in the brain of postoperative aged rats.This will lay a strong foundation for our further study whether Warburg effect mediates the antagonistic effect of H₂ S on POCD.[Methods]The expressions of Warburg effect-related protein,including Hexokinase II HK2),Pyruvate kinase M2 PKM2),Pyruvate dehydrogenase kinase 1 PDK1),Pyruvate dehydrogenase PDH),Lactate dehydrogenase A LDHA)in brain tissues were detected by western blotting.The content of lactate in brain tissues was detected using lactic acid kit.[Results]1.H₂ S upregulated Warburg effect in the brain of postoperative aged rats.Sodium hydrosulfide Na HS,a donor of H₂ S,100 ?mol/kg,i.p.)significantly increased the expressions of HK2,PKM2,LDHA,and PDK1,decreased the expression of PDH,and elevated the level of lactate in the brain of postoperative aged rats.These results indicated that H₂ S ameliorates Warburg effect in the brain of postoperative aged rats.2.2-DG suppressed H₂S-enhanced Warburg effect in the brain of postoperative aged rats2-DG 100 mg/kg,i.p.)markedly inhibited H₂S-elicited upregulation in the expressions of HK2,PKM2,LDHA,and PDK1,downregulation in PDH expression,and increase in lactate content,in the brain of postoperative aged rats.These results indicated that 2-DG inhibits H₂S-enhanced Warburg effect in the brain of postoperative aged rats.[Conclusion]1.H₂ S enhances Warburg effect in the brain of postoperative aged rats;2.2-DG blocks the up-regulatory role of H₂ S in Warburg effect in the brain of postoperative aged rats.