Research Of The Effect On Notch Signaling Pathway In Gastrodin Inhibits Lipopolysaccharide-induced Activation In Microglial Cells

Objective:To explore whether Gastrodin(Gas)inhibited the inflammation in BV-2 cells and the microglial cells of corpus callosum in neonatal rats induced by lipopolysaccharide(LPS)through Notch signaling pathway.And to provide theory gist for the application of Gatrodin in the treatment of neurodegenerative diseases related to neuroinflammation.Methods:In vivtro,we adopted BV-2 cells LPS(1 ?g/ml)stimulated model and divided into control group,LPS(6 h or 2 h)-stimulated group and different concentrations(10,20,50 and 100 ?mol/L)of Gastrodin pretreated(1 h)+LPS co-treated group.The expression of COX-2,iNOS,Notch-1,RBP-J?,Hes-1 mRNAs and COX-2,iNOS,Notch-1,NICD,RBP-J?,Hes-1 proteins were detected by RT-PCR,Western blotting and immunofluorescence staining.In vivo,we randomly choosed 40 healthy neonatal 3-day-old SD rats and divided into control group,LPS(1 mg/kg,6 h)-stimulated group,different doses(50,100 and 200 mg/kg)of Gastrodin pretreated(2 h)+LPS co-treated group.Then we employed RT-PCR,western blotting and immunofluorescence staining to detect COX-2,iNOS,Notch-1,RBP-J?,Hes-1 mRNAs and COX-2,iNOS,Notch-1,NICD,RBP-J?,Hes-1 proteins.Results:In vivtro and in vivo experiment,compared with the control group,the levels of the expression of COX-2,iNOS,Notch-1,RBP-J?,Hes-1 mRNAs and proteins as well as NICD nuclear translocation were significantly up-regulated in the LPS-stimulated group(P<0.05).And the levels of the expression of these above mRNAs and proteins and NICD nuclear translocation in Gastrodin pretreated+LPS co-treated group were down-regulated(P<0.05).Conclusion:Gastrodin can inhibit the inflammatory response induced by LPS in microglia through the Notch signaling pathway.These results provided a theory gist for the application of Gatrodin in the treatment of neurodegenerative diseases associated with neuroinflammation.