Protective Effect Of Gastrodin On LPS-induced Inflammation In PC12Cells

Objective:This study was to investigate the protective effect of gastrodin (GAS) in the lipopolysaccharide (LPS)-stimulated PC12cells.Methods:The inflammatory cell model was structured by LPS-stimulated PC12cells, the cells were treated with GAS (40、60and80μmol/ml)prior to LPS (1mg/ml) exposure. The effects of GAS on viability of PC12cells were reflected by the expressions of tumor necrosis factor (TNF-a) and interleukin-1β (IL-1β), which were tested by double-immunofluorescence labeling assay and reverse transcription polymerase chain reaction (RT-PCR).Results:The results indicate that LPS (lmg/ml,18h)-induced PC12cell inflammatory factor were suppressed by different concentrations of GAS (40、60and80μmol/ml),and the degrees of expression shows a dosedependent.Conclusion:GAS can suppress the release of LPS-induced PC12cell inflammatory factor, thus display its effect of neuroprotective. Arising from the above, we suggest that gas has a potential as an anti-inflammatory drug candidate in neurodegenerative diseases.