The Value Of MRS In Assessing Immunotherapy Effect Of CIK In Human Prostate Colon Cancer Transplanted Subcutaneously In Nude Mice

Objective:To evaluate the effect of magnetic resonance spectroscopy?MRS?on the immunotherapy effect of CIK cells on human subcutaneous allograft of prostate cancer in nude mice.Methods:A total of 48 human prostate cancer models of PC-3 in nude mice were established,and they were randomly divided into CIK immunotherapy group?n=24?and control group?n=24?.CIK immunotherapy group were injected CIK cells with dose 0.2ml 1×10¹⁰/L one time and the control group were injected with the same amount of 0.9%sodium chloride so-lution in the same locations,MRS scans were performed before therapy and 7 days,14 days,21 days after treatment.After MRI scanning,8 mice were sacrificed res-pectively in three time points,and the tumor tissue were taken for pathological ex-amination.The following parameters?Cit?Cho and Cre?which reflecting the tumor metabolism level were observed and?Cho+Cre?/Cit was analyzed.The correlations between the changes of tumor cells apoptosis and?Cho+Cre?/Cit parameter at each time point were analyzed.Results:There were no statistically difference between?Cho+Cre?/Cit of the two groups before treatments.Two groups of 7 days,14 days,21days scan results show that CIK immunotherapy group Cit peak rise gradually,Cho peak gradually decreased,however the control group Cit peak gradually reduce,Cho peak gradually rise,the?Cho+Cre?/Cit ratio reduce,the statistical differences in the two groups were existed?P<0.05?.Compared with the control group,the apoptosis of tumor cells were increased in CIK immunotherapy group.the?Cho+Cre?/Cit ratio of the two groups after treatments were negatively correlated with tumor cells apoptosis at each time point?P<0.05?.Conclusion:CIK immunotherapy has a better inhibitory effect on human prostate cancer transplantation tumor and can accelerate the apoptosis of tumor cells.The parameters obtained by MRS imaging can directly reflect the metabolic level before and after treatment of prostate cancer,and it is of great value to evaluate the efficacy of CIK immunotherapy in prostate cancer.