PLGA Polymer Film Of Liraglutide Immobilization Induced Differentiation Of Islet B-like Cell From Bone Marrow Mesenchymal Stem Cell

Objective:The purpose of this study was to fix Liraglutide(LIR)on poly(lactic-co-glycolic acid)(PLGA)film via dopamine(DA)surface modification method and prepare LIR-DA-PLGA.Further research proved whether LIR-DA-PLGA could induce islet B-like cells(IBCs)derived from bone marrow mesenchymal stem cells(BMSCs).Methods:1.Preparation of PLGA films: PLGA solution evenly spread on silicified silicide and the PLGA films were prepared.LIR was fixed on the PLGA films through DA surface modification(i.e.,LIR-DA-PLGA).At the same time,PLGA films simply modified by DA(i.e.,DA-PLGA)were prepared.2.Extraction,cultivation and differentiation of BMSCs: The bone marrow was extracted from the femurs of rat pubs and cultured in low-glucose culture medium to the third-generation cells.The cells were inoculated respectively onto the LIR-DA-PLGA,DA-PLGA,and PLGA films in high-glucose medium.3.Surface performance of PLGA films: The surface properties of LIR-DA-PLGA,DA-PLGA,and PLGA were detected by contact angle meter,Fourier infrared detector,and scanning electron microscopy(SEM).4.Cell proliferation and growth state: On the first and third days of cell inducted culture,the cell proliferation was detected by methyl thiazolyl tetrazolium(MTT)assay.On the third day of induced culture,the cell growth state was observed by isothiocyanate(FITC)staining.5.Expression of IBCs related proteins and genes: On the seventh day of inducted culture,immunofluorescence staining and real-time quantitative polymerase chain reaction(RT-qPCR)were used to detect the expression of IBCs related proteins and genes of the cells on LIR-DA-PLGA and DA-PLGA films.Results:1.Surface properties of the films: The results of surface properties measurement proved that LIR-DA-PLGA and DA-PLGA were prepared.What's more,the results of contact angle showed that DA and LIR could enhance hydrophilicity.2.Cell proliferation and growth status: After DA surface modification,the cell proliferation rate significantly increased and the cell growth was well.After LIR was fixed by DA surface modification,the cell growth situation depended on LIR concentration and 10mL/L and 20mL/L LIR-DA-PLGA films were more suitable for cell growth.In consideration of economic cost and drug toxicity,10mL/L LIR was selected as the optimal concentration of LIR and 10mL/L LIR-DA-PLGA was prepared as the inducing carrier.3.Expression of IBCs relative proteins and genes: The proteins expression of cells on 10mL/L LIR-DA-PLGA was more than that of DA-PLGA.The genes expression of cells on 10mL/L LIR-DA-PLGA was 2-folds more than that of DA-PLGA.Conclusion:1.DA could promote cell proliferation.LIR could be fixed on PLGA films via DA surface modification method and LIR-DA-PLGA was prepared as induced carrier.2.Fixation of liraglutide was beneficial to BMSCs induced differentiation into IBCs and 10mL/L LIR-DA-PLGA was suitable for induction carriers.