Investigation Of Hyaluronic Acid Modified Cationic Niosomes For Ocular Gene Delivery System

In recent years,taking gene therapy to treat retinal diseases fundamentally has aroused more and more attention by research scholars.However,the bottleneck in gene therapy is the efficient delivering vectors.Therefore,searching for an ideal vector that can tightly bind genes and achieve the goal of locating target tissues,releasing genes on time and performing site-specific self-degradation becomes the most important issue at present.This topic is studied from three parts.Firstly,the study investigated the preparation conditions and physical and chemical properties of Niosomes.After the modification of HA,HA-C-niosomes' size were about 175~184 nm,with a negative potential of-29~-33 mV.Secondly,ARPE-19 cells were used to study the level of cellular uptake.The results showed that the cellular uptake capacity of each group increased with the increase of HA,and the uptake capacity of 20% HA-C-niosomes group was significantly higher than that of the HA-free group.Finally,enhanced green fluorescent protein(pEGFP)was used as plasmid to load cationic liposomes,and modified with hyaluronic acid to obtain ha-c-niosomes /DNA.The internal transfection efficiency was evaluated.In vitro cytological results showed that the transfection efficiency of 20% HA-C-niosomes was increased by 2 times compared with that of the naked plasmid group.In addition,the expression of green fluorescent protein in the RPE layer was detected by the evaluation of transfection in the body of SD rats.And the transfection efficiency of 20%HA-C-niosomes group was 6.5 times of the naked plasmid group.In summary,the HA-modified cationic liposomes(HA-C-niosomes)have good stability and gene protection,can proactively target RPE cells and achieve good gene transfection.