The Effects Of Ghrelin On Opoptosis And Fractalkine Expression In Human Umbilical Vein Endothelial Cells Induced By High Glucose

ObjectiveTo explore the effects of ghrelin on the apoptosis and fractakine?FKN?expression in human umbilical vein endothelial cells induced by high glucose.MethodsHUVECs were clutrued in EMEM/LG 5.5mmol/L)for 10 h,10^-7mol/L ghrelin was added and incubated for 2h and then incubated with media with33.3mmol/L glucose for 48h.The apoptosis of HUVECs was evaluated by flow cytometry?FCM?and through Hoechst 33258 straining.Expression of FKN was detected by Q-RT-PCR.Protein level of FKN was determined by western blot.ResultsHigh glucose significantly induced an increase in apoptosis in HUVECs which was partly inhibited by ghrelin?P=0.000?,though the apoptosis of HG+ghrelin still higher than normal control?P=0.000?.Compare with normal control,HG enhanced the fractalkine expression at both protein?P=0.000?and mRNA?P=0.001?levels,and both the protein and mRNA levels of FKN were decreased in HG+ghrelin?P=0.000?.ConclusionGhrelin reduces the apoptosis rate of HUVECs in high glucose environment,the mechanism may be related with ghrelin intervation in inhibiting the expression of FKN in HUVECs.