The Effects Of Sodium Tanshinone Ⅱa Sulfonate Pretreatment On High Glucose-induced Expression Of FRACTALKINE And Apoptosis In Human Umbilical Vein Endothelial Cells

Objective: To explore the expression of fractalkine (FKN) and the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by high glucose and its possible relationship with Wnt signaling pathwat.Methods: HUVECs in good condition were divided into 4 groups : normal group (N), high glucose group (HG), lithium chloride group (LiCl), high glucose with lithium chloride group (HG+LiCl). After planted HUVECs in DMEM/LG(5.5mmol/L) for 10h,10mmol/L LiCl was added for 2h as above and then 33.3mM high glucose was added for 48h.The apoptosis of HUVECs were evaluated by TUNEL assay, the protein 1evels of intracellularβ-catenin and p-GSK-3β(Ser9) were measured by immunohistochemistry staining(SABC) and fractalkine were detected by immunofluorescence(FITC), and the mRNA expression of GSK-3βand FKN were measured by RT-PCR.Results: 1.High glucose induced apoptosis rate of HUVECs higher than N (P<0.05) significantly and it could be partly inhibited by HG+LiCl (P<0.05),but the apoptosis of HG+LiCl still higher than N(P<0.05). 2.Compare with N, HG suppressed the protein expression ofβ-catenin and p-GSK-3β(Ser9) (P<0.05), increased the mRNA levels of GSK-3β(P<0.05) and enhanced the fracalkine expression at both protein and mRNA(P<0.05) levels. 3. Compare with HG, the protein levels ofβ-catenin and p-GSK-3β(Ser9) were improved (P<0.05), and the mRNA levels of GSK-3β(P<0.05) and both the protein and mRNA(P<0.05) levels of fractalkine were decreased in HG+ LiCl.Conclusion: The apoptosis of HUVECs induced by high glucose may be relevant to the inhibition of Wnt signaling pathway and the upregulation of fractalkine levels. Objective: To explore the effects of sodium tanshinoneⅡA sulfonate(STS) pretreatment on high glucose-induced ecpression of fractalkine(FKN) in human umbilical vein endothelial cells (HUVEC) and its possible mechanisms.Methods: HUVECs in good condition were divided into 5 groups : normal group (N), high glucose group (HG), STS group (STS), high glucose with STS group (HG+STS) and high glucose with STS and lithium chloride group (HG+STS+LiCl). After planted HUVECs in DMEM/LG (5.5mmol/L) for 10h, 5mg/L STS and/or 10mmol/L LiCl was added for 2h as above and then 33.3mM high glucose was added for 48h.Harvested and the protein 1evels of intracellularβ-catenin and p-GSK-3βwere measured by immunohistochemistry staining (SABC) and membrane fractalkine were detected by immunofluorescence (FITC), and the mRNA expression of GSK-3βand FKN were measured by RT-PCR.Result: Compare with N, the mRNA levels of GSK-3β(P<0.05) and both the protein and mRNA level (P<0.05) of fracalkine were increased and the protein expression ofβ-catenin and p-GSK-3β(Ser9) (P<0.05) were decreased in HG; Compare with HG, the mRNA levels of GSK-3β(P<0.05) and both the protein and mRNA levels (P<0.05) of fractalkine were decreased and the protein levels ofβ-catenin and p-GSK-3β(Ser9) were improved (P<0.05) in HG+STS; and both STS and lithium chloride pretreatment enhanced the effects above(P<0.05).Conclusion: STS pretreatment suppress the expression of FKN in HUVEC induced by high glucose and it may be relevant to Wnt signaling pathway.