Association Of Macrophage Migration Inhibitory Factor Gene Polymorphism And Abnormal Expression With Gestational Diabetes Mellitus

Objective:To investigate the correlation between macrophage migration inhibitory factor(MIF)gene polymorphism(SNP)and abnormal expression of MIF in placental tissues and the pathogenesis of gestational diabetes mellitus(GDM).Method:In a case-control study,PCR-RFLP was used to detect MIF-755622 site SNP in peripheral blood of 224 GDM patients and 260 healthy pregnant women,and Western-Blot was used to detect the expression of MIF in placenta tissue of 126 GDM patients and 150 healthy pregnant women,the differences of blood glucose,insulin levels and clinical physical and chemical indexes among different genotypes were detected and analyzed.Result:(1)The CC+CG genotype frequencies of MIF-755622 in GDM group were higher than that of GG genotype(c~2=15.46,P<0.001),the allele frequencies of C were higher than those of controls(c~2=16.38,P<0.001).(2)Compared with GG genotype,the fasting glucose(FBG),OGTT(1h),OGTT(2h)and insulin resistance index(HOMA-IR)of MIF-755622 genotype in CC+CG group were significantly higher than that in GDM group(P<0.001).(3)The relative expression of MIF in placenta tissue of GDM group(0.85±0.10)was higher than that in control group(0.12±0.09,P<0.001).(4)In GDM group,the expression of MIF protein in placenta was positively correlated with FPG and HOMA-IR(r=0.378,0.469,P<0.001).(5)The relative expression of MIF-755622 in CC+CG genotype in placenta tissue(1.29±0.64)was higher than that in GG genotype pregnant women(0.87±0.52).Conclusion:The correlation between SNP and GDM in the MIF-755622 site was correlated with the degree of insulin resistance(IR)in GDM pregnant women.At the same time,SNP of this site is associated with the up regulation of MIF expression in placenta tissue of GDM group,and plays an important role in the occurrence and development of GDM.