The Clinical Significance Of CUL4A In Breast Cancer

Objectives:To detect the expression of CUL4A,E-cadherin and p53 in breast cancer and adjacent normal breast tissue and to focus on the expression of three proteins in mammary duct carcinoma,and to provide a new theory for the clinical treatment of mammary duct carcinoma.Methods:Immunohistochemical method was performed to detect the expression of CUL4A,E-cadherin and P53 in 15 cases of adjacent normal breast tissue,16 cases of ductal carcinoma in situ and 69 cases of invasive breast cancer(including 62 cases of IDC and 7cases of ILC).Results:1.The positive rate of CUL4A in breast cancer and adjacent normal breast tissue was85.5%(59/69)and 6.7%(1/15),and the positive expressive intensity of CUL4A in breast cancer is higher than that in adjacent normal breast tissue(Z=5.202,P<0.05).The positive rate of CUL4A in IDC and ILD was 83.9%(52/62)and 100%(7/7),and and there was no significant difference in positive expression(Z=0.993,P>0.05).The positive rate of E-cadhein in breast cancer and adjacent normal breast tissue was 81.2%(56/69)and100%(15/15),and the positive expressive intensity of E-cadherin in breast cancer is lower than that in adjacent normal breast tissue(Z=4.9351,P<0.05).The positive rate of E-cadhein in IDC and ILD was 90.3%(56/62)and 0%(0/7),and the positive expression difference was statistically significant(Z=3.751,P<0.05).The positive rate of P53 in breast cancer and adjacent normal breast tissue was 53.6%(37/69)and 0(0/15),and the positive expressive intensity of P53 in breast cancer was higher than that in adjacent normal breast tissue(Z=3.599,P<0.05).The positive rate of P53 in IDC and ILD was53.2%(33/62)and 57.1%(4/7),with no significant difference in positive expression(Z=0.351,P>0.05).2.The positive rate of CUL4A in adjacent normal breast tissue,DCIS and IDC was6.7%(1/15),56.3%(9/16)and 83.9%(52/62),and the difference was statistically significant in three groups(X~2=33.226,P<0.05).In both comparisons,the positive expression intensity of CUL4A in IDC was higher than that in DCIS and adjacent normal breast tissue(Z=3.495,5.038;P<0.05).The positive expression intensity of CUL4A in DCIS was higher than that in adjacent normal breast tissue(Z=2.916,P<0.05).The positive expression of CUL4A in IDC was related to axillary lymphnode metastasis,TNM staging,and histological grade(Z=3.172,2.975,3.144;P<0.05),but not to the age and tumor size of patients(Z=0.102,1.912;P>0.05).3.The positive rate of P53 in adjacent normal breast tissue,DCIS and IDC was 0(0/15),18.8%(3/16)and 53.2%(33/62),and there was a statistical significance in three groups(X~2=17.061,P<0.05).In both comparisons,the positive expression intensity of P53 in IDC was higher than that in DCIS and adjacent normal breast tissue(Z=2.484,3.558;P<0.05).There was no statistical difference in positive expression intensity between P53 in DCIS and adjacent normal breast tissue(Z=1.734,P>0.05).The positive expression of P53 in IDC was related to axillary lymphnode metastasis(Z=2.353,P<0.05),but not to patient’s age,the size of cancer,histological grade and TNM staging(Z=0.662,1.336,0.404,0.809;P>0.05).4.The positive rate of E-cadherin in adjacent normal breast tissue,DCIS and IDC was 100%(15/15),100%(16/16)and 90.3%(56/62),and there was a statistical significance in three groups(X~2=25.928,P<0.05).The positive expression intensity of E-cadherin in IDC was lower than that in DCIS and adjacent normal breast tissue(Z=2.366,4.776;P<0.05).The positive expression intensity of E-cadherin in DCIS was lower than that in adjacent normal breast tissue(Z=3.095,P<0.05).The positive expression of E-cadherin in IDC was related to axillary lymphnode metastasis and TNM staging(Z=3.456,3.302;P<0.05),but not to patient’s age,the size of cancer and histological grade(Z=0.481,0.735,1.443;P>0.05).5.The expression of CUL4A was negatively correlated with E-cadherin in IDC(r=-0.536,P<0.05),but there was no correlation between CUL4A and P53(r=0.119,P>0.05).Conclusions:1.CUL4A,P53 and E-cadherin have a certain relationship with the occurrence of breast cancer.2.There was a significant difference in the expression of E-cadherin between IDC and ILC.There was no significant difference in the expression of CUL4A and P53between IDC and ILC.3.CUL4A,P53 and E-cadherin promote the occurrence,development,infiltration and metastasis of breast ductal carcinoma.4.In breast ductal carcinoma,CUL4A negatively regulates the expression of E-cadherin.