| OBJECTIVE:Previous studies have found that there are different levels of sleep disorders in patients with Chronic Fatigue Syndrome(CFS).Acupuncture has an obvious adjustment effect on CFS with sleep disorders,but its mechanism needs to be deepened.In this study,we use CFS model rats as the research carrier to explore: 1.the effect of acupuncture on sleep disorders in rats with CFS sleep disorder model,and 2.acupuncture on the mechanism of adenosine and its receptor in the sleep adjustment effect of CFS sleep disorder model rats.Methods:1.Effect of acupuncture on sleep disturbance in CFS sleep disorder model rats30 SD rats were domesticated by 7 days for 1 cycles,and rats with better environmental adaptability were selected.The rats were randomly divided into normal group(A group),model group(hereinafter referred to as B group)and model acupuncture group(hereinafter referred to as C group)by DPS treatment system.Rats in group B and group C were subjected to 21 days' modeling by exhaustive swimming plus chronic restraint.After the model was finished,group A was not treated with any treatment.After third days of success,group B began to use self-made fixtures to fix rats,fixed time for 15 minutes,once a day for 2 days after 5 days of continuous treatment,and group C was given acupuncture for "hundred meeting","Shenmen","Shenshu","Shenshu","spleen Yu","after three li".Treatment,time and frequency of the same group B.Before and after treatment,8:00-20:00 was used to monitor sleep time and sleep locations of SD rats.The behavioral indexes of SD rats were tested by exhaustive swimming,elevated cross maze test,open field test and water maze test.2.The effect of acupuncture on the basal forebrain adenosine and its receptor in rats with CFS sleep disorder(1)The study of the effects of experiment one and acupuncture on adenosine and receptor in the basal forebrain of CFS sleep disorder model rats: modeling method,grouping and treatment plan before the same.After acupuncture treatment,the content of adenosine in basal forebrain of rats in each group was detected by ELISA,and the corpus striatum and ventral lateral hypothalamus in the basal forebrain were detected by immunohistochemistry The content of A1 receptor in the preoptic area,and thecontent of A2 a receptor in striatum and nucleus accumbens region.(2)The effect of acupuncture on adenosine and receptor in the basal forebrain of rats with CFS sleep disorder model after intraperitoneal injection of adenosine receptor antagonist was studied in experimental two,CFS sleep disorder model rats.The rats were domesticated for 7 days for 7 days,and the rats with better environmental adaptability were selected.Using DPS software,rats were randomly divided into two methyl sulfoxide(dimethyl sulphoxide,DMSO)group(hereinafter referred to as D group),DMSO+ acupuncture group(hereinafter referred to as E group),A1 receptor antagonist + acupuncture group(hereinafter referred to F group),A2 receptor antagonist + acupuncture group(hereinafter referred to as G group).Rats in each group were subjected to exhaustive swimming and chronic restraint to reproduce CFS animal models.After the model was finished,DMSO was injected into the abdominal cavity of rats in group D and group E for 7 days,and the effect of DMSO itself on rats was excluded.F and G groups were injected with adenosine A1 and A2A receptor antagonist in abdominal cavity according to 0.125ml/100 g and0.105ml/100 g.After 7 days of injection,group D was not treated with any treatment.Group E,F and G were treated with "Baihui","Shenshu","Shenshu","Shenshu","spleen Yu" and "after three li".The treatment time was 15 minutes a day,once a day for 5 days after 2 days of rest.After the treatment,the content of adenosine in the basal forebrain of the rats was detected by ELISA,and the content of A1 receptor in the preoptic region of the basal forebrain and the ventral lateral hypothalamus,and the content of A2 a receptor in the striatum and the nucleus accumbens were detected by immunohistochemistry.Results:1.Effect of acupuncture on sleep disturbance in CFS sleep disorder model ratsAfter successful CFS modeling,compared with group A,the sleep time of group B and group C was significantly shortened,and the location of sleep was obviously not fixed,the difference was statistically significant(P<0.01).After acupuncture,compared with group B,the sleep time of group C was significantly increased,and the sleep site was significantly fixed,with statistically significant difference(P<0.01).2.The effect of acupuncture on the basal forebrain adenosine and its receptor mechanism of sleep adjustment in CFS sleep disorder rats(1)Study on the effects of experiment one and acupuncture on adenosine and receptor in the basal forebrain of CFS sleep disorder model rats: after the success ofCFS model,the content of adenosine in the basal forebrain of group B decreased significantly,the content of A1 receptor in the striatum and the ventral ventral ventral region of the hypothalamus decreased significantly,and the content of A2 a receptor in the striatum and the nucleus accumbens nucleus The difference was statistically significant(P<0.01).After acupuncture treatment,the content of adenosine in the basal forebrain of group C was significantly higher than that of the B group.The content of A1 receptor in the striatum and the ventral ventral lateral hypothalamus increased significantly.The content of A2 a receptor in the striatum and nucleus accumbens was significantly higher than that in the striatum and the nucleus accumbens(P<0.05).(2)The effect of acupuncture on adenosine and receptor in the basal forebrain of rats with CFS sleep disorder model after intraperitoneal injection of adenosine receptor antagonist by intraperitoneal injection of adenosine receptor antagonist in rats of experimental two and CFS sleep disorder model: after acupuncture treatment with adenosine receptor antagonist intraperitoneally,the concentration of adenosine in the basal forebrain of group E was significantly higher than that in group D,and the difference has a significant difference.There were statistical significance(P<0.01).Compared with the E group,the concentration of adenosine in the basal forebrain of the F group decreased significantly,and the A1 protein content in the ventral anterolateral hypothalamus decreased significantly(P<0.01),and the content of A2 a protein in the nucleus accumbens was reduced,and the content of A2 a protein in the ventral striatum was not significantly changed,and the difference was not statistically significant.Meaning(all P>0.05).Compared with the E group,the concentration of adenosine in the basal forebrain of the G group decreased significantly,and the A2 a protein content in the ventral striatum decreased significantly(P<0.01).The A2 a protein content in the nucleus accumbens was reduced,and the A1 protein in the ventral anterolateral hypothalamus had no significant difference(P >0.05).Compared with the F group,the content of A1 protein in the ventral anterior region of the hypothalamus increased significantly in the G group,and the content of the A2 a protein in the ventral striatum decreased significantly(P<0.01),and the A2 a protein content in the nucleus accumbens was decreased,and there was no statistical difference(P>0.05).Conclusion:1.CFS model rats had sleep disorders,characterized by significantly reducedsleep time and unfixed sleep locations.Acupuncture treatment significantly increased sleep time and fixed sleep locations in CFS rats.2.The adjustment of CFS's sleep disorder may be through the release of adenosine from the basal forebrain,the A1 receptor in the striatum,the ventral ventral hypothalamus,and the A2 a receptor in the striatum and the nucleus accumbens. |
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