| Objective:To further investigate and discuss the central mechanism of acupuncturing the "Neiguan" point to regulate sleep-wake cycle through observing effects of sleep-wake cycle, adenosine content in the basal forebrain, expressions of adenosine A1 and A2a receptors in hippocampus of acupuncture on insomnia rats so as to provide theoretical basis for treating sleep-wake cycle disorders related diseases in clinic.Methods:After domesticating SD rats under a normal light-dark cycle,32 rats with well light-dark cycle adaptation were picked up and randomly divided into a normal saline (NS) group (n=8) and a model replication group (24 rats) in accordance with the random number table. The model copy group was randomly divided into a model control group(n=8), a binding group(n=8) and an acupuncture group (n=8) in accordance with the random number table after establishing PCPA insomnia models.The saline group and model control group were untreated. The bundled group was only bundled from the third day after PCPA model established for five consecutive days, once per day and every time lasted 15 minutes. The acupuncture group was accepted treatment of acupuncturing the "Neiguan" point following the same time arrangement and frequency of the bundled group.The EEG waves were monitored and analyzed using physiological telemetry system; The adenosine content in basal forebrain in each group were analyzed using microdialysis plus high performance liquid chromatograph (HPLC); and adenosine Al and A2a receptors in hippocampus were detected by using immunohistochemistry.Results:1.After PCPA induced insomnia model established, the wake time has been increased significantly, while the total sleep time has been decreased in 24 hours in the model replication group. In which, compared with the saline group, the difference was statistically significant (P<0.05)2.After acupuncturing the "Neiguan" point, the wake time has been obviously reduced and the total sleep time has been increased significantly in the acupuncture group. Compared with the model control group and the bundled group, the difference was statistically significant (P<0.05),and there was no statistical difference while compared with the saline group (P>0.05).3.After PCPA induced insomnia model established, adenosine content in the basal forebrain was increased and positive expression of Al receptors in the hippocampus were increased in the model control group, in which compared with the saline group, there was significant difference (P<0.05). Positive expression of adenosine A2a receptors in the hippocampus in the model control group was not significantly different from that in the saline group (P>0.05).4. After acupuncturing the "Neiguan" point, adenosine content in the basal forebrain in the acupuncture group was significantly reduced, in which there was significant difference while compared with the model control group (P<0.05) and there was no statistical difference while compared with the saline group (P>0.05).5. After acupuncturing the "Neiguan" point, positive expression of A1 receptors in the hippocampus in the acupuncture group was decreased compared with the model control group and bundled group. And the difference was statistically significant (P<0.05). When compared with the saline group, there was no statistical difference. There was no statistically significant difference on positive expression of adenosine A2a receptors between the model control group and saline group (P>0.05)Conclusion:1. Acupuncture the "Neiguan" point can promote the recovery of sleep-wake cycle in insomnia rats;2. The mechanism of acupuncture the "Neiguan" point adjusting sleep-wake cycle on insomnia rats may be related to reduce adenosine content in the basal forebrain area and decrease the expression of adenosine Al receptors in the hippocampus by acupuncture;3. Adenosine A2a receptors in the hippocampus were not involved in acupuncturing the "Neiguan" point to regulate sleep-wake cycle in PCPA induced insomnia rats. |
PDF Full Text Request