| Objective: Polygoni Multiflori Radix set up by using the HPLC fingerprint,principal component analysis and clustering analysis,the analysis of Polygoni Multiflori Radix origin,main ingredients,processing time,etc.,use of clinical drug delivery time determine 28 d Polygoni Multiflori Radix security.Provide a reference for Polygoni Multiflori Radix courses and dosage.Method: Using HPLC method research of 21 different market sale system characteristic of main composition content in radix polygoni multiflori,composition and characteristics,and the assessment of the quality of radix polygoni multiflori different market system,establish a system of quality evaluation of radix polygoni multiflori standards.Up 48 h Polygoni Multiflori Radix aqueous extract of chemical composition were analyzed,and the obtained data with SPSS21.0,A traditional Chinese medicine(TCM)fingerprint computer aided similarity evaluation software version for processing analysis,using PCA and OPLS-DA identify Polygoni Multiflori Radix composition 6 h difference;Component differences and blood biochemical indexes correlation analysis,to find birth/Polygoni Multiflori Radix composition indexes in water extract.Polygoni Multiflori Radix aqueous extract(PERW)and system Polygoni Multiflori Radix aqueous extract(PEPW)different doses for 28 d to 40,20,10 g/kg dose lavage normal SD rats respectively,1 times a day for 28 d observation rat’s general status,changes in body weight,blood biochemical index and the histopathologic examination of the kidney.Comparison of Polygonum Multiflorum Thunb on the rat liver toxicity of raw product and its processed products.Results : The system of 21 group of radix polygoni multiflori get five principal components through principal component analysis,its cumulative variance contribution rate by 81.251%;Clustering analysis method is made the first batch of 21 samples according to the composition characteristics of 4 class,each component contribution rate up to 80% of the ingredients of clustering analysis,get the clustering figure is consistent with the overall composition.Processing Polygonum Multiflorum Thunb 48 h Polygonum Multiflorum Thunb,the content of the chemical composition to 4 ~ 5 h Polygonum Multiflorum Thunb peak,as the processing time extended,component content showed a trend of decline.Clustering analysis method will be 6 h radix polygoni multiflori and radix polygoni multiflori samples according to the composition of basic characteristics of 4 class,partial least squares analysis diphenylethylene glycosides and resveratrol for different processing time the component content of the main differences between the composition change.PERW and PEPW high doses have significant effects on female rats weight,low dose of general situation of SD rats and has no obvious effect on body weight;No obvious difference was found between the pathological tissue;Basic normal liver tissue.Clustering analysis according to the characteristics of biochemical indicators into three classes,from 15 biochemical index index of contribution rate of 75% in the seven AST,ALT,r-GT,GLO,TG,GLU,associates and clustering analysis,get consistent with 15 biochemical index of clustering figure.Conclusion: 21 made the first batch of the variation in content of main component differences;Catechins,aloe emodin-8-o-beta-D-glucoside,giant knotweed glycosides,styrene glycosides,resveratrol and emodin 8-o-beta-D-glucoside,emodin methyl ether glycosides,aloe emodin as the main characteristic components,baoding in hebei and sichuan dabashan mountain sale of radix polygoni multiflori quality better.At 4 h best processing time.The same dose of raw radix polygoni multiflori and radix polygoni multiflori to 28 d,no organic lesion of liver tissue,but related to the metabolism of liver blood biochemical indicators have obvious changes,AST,ALT,r-GT,GLO,TG,GLU,associates can be used as a raw radix polygoni multiflori and radix polygoni multiflori characteristic biochemical indicators of liver toxicity. |
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