Study On The Association Of BOP1 And PEBP4 With Hepatitis B Virus-Related Hepatocarcinoma

Viral hepatitis type B is a disease caused by hepatitis B virus infection.As early as 1970 s,some scholars found that hepatocarcinoma(HCC)was associated with chronic hepatitis B virus infection.At present,the diagnostic accuracy of biomarker against chronic hepatitis B related liver cancer is low,and more new studies are still needed to find new biomarkers for early detection of disease progression.The hepatocarcinoma caused by Hepatitis B Virus(HBV)infection involves the interaction and regulation of various genes,transcriptional transcripts and proteins.Prior to this study,other members of our groups had screened HBV-related liver cancer genes and further explored whether they mightspecifically be used as a biomarker to detect variousdevelopment stages of HCC.In practical clinic application,new biomarkers or drug targets would help the early diagnosis and treatment of HCC.First,the related genes of hepatocellular carcinoma were screened by bioinformatics tools such as WGCNA.From the selected results,the gene BOP1 and PEBP4 were found to be related to the development of liver cancer.In this study,we investigated the effects of the both gene expression levels on the HepG2 cells to determine their potential relationship with HBV-related hepatocarcinoma.In the present twork,we successfully constructed plasmids pcDNA3.1-BOP1 and pcDNA3.1-PEBP4 for intracellular over-expression exploration,as well as interference plasmids shBOP1 and shPEBP4,then successfully transfected them into HepG2 cell line,and modulatedthe regulation of mRNA and protein level.After transfection of 72 h,the expression level of BOP1 within pcDNA3.1-BOP1 transfected cells was up to 8 times and the expression level of BOP1 within shBOP1 transfected cells was down to 0.8 times;meanwhile,the expression level of PEBP4 within pcDNA3.1-PEBP4 transfected cells was up to 15 times and the expression level of PEBP4 within shPEBP4 transfected cells was down to 0.9 times.We further studied the effect of the alterationof gene expression levels on HepG2 cell behavior.The results showed that the over-expression of BOP1 promotes cellular proliferation and migration,while the down-regulation of BOP1 repressesthe proliferation and migration;the over-expression of PEBP4 also promotes the proliferation and the migrationafter transfection of 48 h.The down-regulation of PEBP4 inhibits the cellular proliferation and migration.The interference of BOP1 and PEBP4 leads to apoptosis of HepG2 cells.