Protective Effects Of Alpha-mangostin On Human Retinal Pigment Epithelium Cells From Oxidative Damage

Background:Age-related macular degeneration?AMD?is a common cause of irreversible vision loss among elderly people,which affects the macula.As the population aging in our country,the prevalence of AMD is increasing.Although the pathogenic mechanism of AMD is poorly understood,it is a multifactor disease which can be affected by individual and environmental factors.Studies have shown that oxidative stress has an important role in the pathogenic mechanism of AMD,and studies have confirmed that pathologic damage induced by oxidative damage to retinal pigment epithelial?RPE?cells is an important event in AMD.RPE cells has many important physiological functions,it is located in the outermost layer of retina with lots of metabolic activity.In many diseases such as AMD,retinal light damage and so on,it is susceptible to oxidative damage.Alpha-mangosteen?a-Mangostin?,is a quinonoid constituent isolated from mangosteen?Garcinia mangostana Linn?,which have important biological activity such as anti-oxidant,anti-inflammatory,anti-bacterial,anti-cancer and so on.Objective:To study of a-Mangostin protective effects in RPE cells from oxidative damage induced by hydrogen peroxide?H₂O₂?.Methods:RPE cells were randomly divided into normal control group,oxidative damage group?H₂O₂ induced group?and a-Mangostin treatment groups.And?-Mangostin treatment groups were divided into subgroups according to the different concentrations of?-Mangostin.CCK8 method was used to detect Cell viability changes in each group.The expression of Reactive Oxygen Species?ROS?level was detected by flow cytometry?FCM?and the expression of transcription factor nuclear factor-Erythroid-2-related factor 2?Nrf2?,Heme oxygenase-1?HO-1?,NADH Dehydrogenase,Quinone 1?NQO1?and nuclear factor-kappaB?NF-kB?Protein was detected by Western blot analysis.Results:CCK8 examination results showed that:within 0-12?M,?-Mangostin had no damage effects on cell activity compared with normal control group.After H₂O₂induced,the activity of RPE cells in oxidative damage group significantly reduced compared with normal control group,P<0.05.And compared with oxidative damage group,cell viability of?-Mangostin treatment groups gradually increased when the concentrations of?-Mangostin were within 0 to 12?M.ROS results showed:compared with normal control group,the expression of ROS level significantly increased in oxidative damage group?P<0.05?and the expression of ROS level in12?M?-Mangostin treatment group decreased compared with oxidative damage group?P<0.05?.Western blot results showed that oxidative damage group showed a positive expression of Nrf2,HO-1,NQO1,NF-kB protein compared with normal control group,P<0.05.Compared with oxidative damage group,the expression of Nrf2,HO-1,NQO1,NF-kB protein in 12?M?-Mangostin treatment group increased?P<0.05?.Conclution:H₂O₂ induced oxidative damage in RPE cells by decreasing cell viability and increasing the expression of ROS level.?-Mangostin can protect RPE cells from the injury of H₂O₂,the mechanism may be related to the clear of ROS and the up-regulation of Nrf2,HO-1,NQO1,NF-kB expression.