Study Of Metabolite Groups Of Polygonum Cuspidatum Extract In Rat Plasma Using Fe₃O₄@SiO₂ Magnetic Molecularly Imprinted Polymers Coupled With LC/Q-TOF-MS

Traditional Chinese medicine(TCM)medicinal material basis plays an important role in the pharmacological activities research,quality control and development and application of TCM.In recent years,the research of metabolites groups is hot topic to illustrate the efficacy material basis.However,because of the complex constituents,multiple components,low concentrations of metabolites and matrix interference,analysis of effective substances or active metabolites groups of TCM have been a big challenge.By magnetic nanotechnology,dendritic polymers and the study of the surface molecular imprinting technology,the aim of paper is to establish a reliable method of magnetic molecular imprinting polymer dispersive solid-phase extraction(MMIPs-DSPE)for specific recognition and selective enrichment of metabolite groups(stilbenoids and anthraquinones)of Polygonum cuspidatum extract in rat plasma coupled with LC/Q-TOF-MS.With the dendritic-grafting modification,magnetic nanoparticles(MNPs)possessed the outstretched branched structure,orderly open cavities and abundant functional groups with a good specific surface area(47.99 m2 g-1).PD-MMIPs and EG-MMIPs were obtained using polydatin(PD)and emodin-8-O-β-D-glucoside(EG)as template molecules,respectively,methacrylic acid as functional monomer,ethylene glycol dimethacrylate as cross-linker and 2,2’-azobisisobutyronitrile as initiator.The membrane thickness of both MMIPs obtained from scanning electron microscopy was approximately 10 nm.And both MMIPs obtained high adsorption capacity(221 μmol g-1 for PD and 190μmol g-1 for EG)and fast kinetics.Dispersion solid-phase extraction using MMIPs was applied to selectively separate and enrich the target metabolite groups of stilbenoids and anthraquinones in rat plasma,respectively.Furthermore,three classical pretreatment methods including protein precipitation(PP),liquid-liquid extraction(LLE)and solid phase extraction(SPE)were compared with MMIPs-DSPE in terms of matrix effect,efficiency of extraction and capacity using LC/Q-TOF-MS.The result showed that MMIPs obtained the lowest matrix effect from 1.52 to 8.96%and the most constituents of target metabolite groups with 17 stilbenoids and 19 anthraquinones,respectively.The information showed that MMIPs had good selective separation and enrichment of target metabolite groups from the complex biological samples.