Effects Of ?-dihydroartemisinin-emodinon Experiment In Human Cervical Carcinoma Hela Cells

Objective:To investigate programmed cell death?proliferation and migration induced by ?-dihydroartemisinin-emodin in human cervical carcinoma Hela cell line.Methods:Human cervical carcinoma Hela cell line was treated by?-dihydroartemisinin-emodin at different concentrations and for different time lengths.MTT assay was used to evaluate the cell inhibition rate to determine the optimal exposure time and concentration.The cells were observed for programmed cell death using HE staining,Hochest33258 staining,AO/EB staining,and morphological changes after being treated by ?-dihydroartemisinin-emodin.Scratch wound migration assay,flow cytometry,Western Blottingting and immunocytochemistry examination the expression levels of the apoptin Caspase-3,Caspase-8,Caspase-9,anti-apoptosis protein Bcl-2,proliferating antigen Ki-67 and Death receptor protein Fas.Results:?-dihydroartemisinin-emodin expression significantly inhibited Hela cell proliferation;significantly promoted Hela cell apoptosis(P<0.01);remarkably decreased Hela cells' migratory ability(P<0.01);Western Blottingting and immunocytochemistry revealed significantly increased of Caspase-3,Caspase-8,Caspase-9,Fas and down-regulation of Ki-67 and Bcl-2(P<0.01).Conclusion:1.The effect of ?-dihydroartemisinin-emodin on the inhibition of proliferation in Hela cell was related to Ki-67;2.The significantly inhibit the growth of Hela cell and induces apoptosis in Hela cell was related to an arrest G1 phase;3.?-dihydroartemisinin-emodin induces apoptosis in Hela cell through intrinsic pathway and extrinsic pathway;4.?-dihydroartemisinin-emodin remarkably decreased Hela cells' migratory ability.