Effects Of Aloe-Emodin On Proliferation, Apoptosis And Cycle Of Human Nasopharyngeal Carcinoma Cell Line CNE2

Objective:The different concentrations of AE(aloe emodin)were acted on human nasopharyngeal carcinoma cell line CNE2.MTT assay and flow cyto-metry were used to detect the effects of AE on proliferation,apoptosis and cycle dis tribution of human nasopharyngeal carcinoma cell line CNE2,to provide the e-xperiment a basis for the clinical application of AE.Methods:The effects of different concentrations of AE on proliferation,apoptosis and cycle of human nasopharyngeal carcinoma cell line CNE2 were observed by MTT assay and flow cytometry.(1)MTT assay: The different concentrations of AE(0ug/ml,2.5ug/ml,5ug/ml,10ug/ml,20ug/ml,40ug/ml)were acted on human nasopharyngeal carcinoma cell line CNE2 for 24 h,48h and 72 h respectively,so as to observe the effects of AE on proliferation of human nasopharyngeal carcinoma cell line CNE2.(2)Flow cytometry: The different concentrations of AE(0ug/ml,2.5ug/ml,5ug/ml,10ug/ml,20ug/ml,40ug/ml)were acted on human nasopharyngeal carcinoma cell line CNE2 for 48 h to observe the effect of AE on apoptosis and cycle distribution of human nasopharyngeal carcinoma cell line CNE2.Results:(1)MTT assay: After the different concentrations of AE(0ug/ml,2.5ug/ml,5ug/ml,10ug/ml,20ug/ml,40ug/ml)were acted on human nasopharyngeal carcinoma cell line CNE2 for 24 h,48h and 72 h,the inhibition rate of cell proliferation increased with the increase of drug concentration and time,and reached the plateau at 40ug/ml.Compared with the blank control group,the experimental group was statistically significant(P < 0.05).The results showed that AE can inhibit the proliferation of human nasopharyngeal carcinoma cell line CNE2 at a concentration of2.5ug/ml-40ug/ml in a time and concentration dependent manner.(2)Cell apoptosis detected by flow cytometry: After the different concentrations of AE(0ug/ml,2.5ug/ml,5ug/ml,10ug/ml,20ug/ml,40ug/ml)were acted on human nasopharyngeal carcinoma cell line CNE2 for 48 h,the results showed that with the increase of drug concentration,the apoptosis rate increased from 1.153% to 3.753%.Compared with the blank control group,it was statistically significant(P<0.05).It showed that the apoptosis rate of human nasopharyngeal carcinoma cell line CNE2 was significantly increased and the concentration was dose-dependent when the AE acted for 48 h.(3)Cell cycle detected by flow cytometry: After the different concentrations of AE(0ug/ml,2.5ug/ml,5ug/ml,10ug/ml,20ug/ml,40ug/ml)were acted on human nasopharyngeal carcinoma cell line CNE2 for 48 h,the results showed that:In the G2/M phase,compared with the AE 0ug/ml group,the proportion of G2/M cells in the other groups were significantly increased.The results indicated that the CNE2 cycle of human nasopharyngeal carcinoma cells was blocked in the G2/M phase when the AE acted for 48 h.Conclusions:1.AE can inhibit the proliferation of human nasopharyngeal carcinoma cell line CNE2,and it is in a time and concentration dependent manner.2.When the AE acted for 48 h,the apoptosis rate of human nasopharyngeal carcinoma cell line CNE2 was not high on the whole.However,there was a tendency to increase in turn,especially the apoptosis rate in the early stage was significantly concentration-dependent.3.The CNE2 cycle of human nasopharyngeal carcinoma cells was blocked in the G2/M phase when the AE acted for 48 h.