Study On The Material Basis Of Cistanche Deserticola Phytoestrogen Antagonism

Traditional Chinese medicine has an important effect on human health and reproduction as a treasure of Chinese culture.Because of its complexity ingredients of Chinese medicine,unknown active component,lack of reliable toxicology,pharmacology research data,hampering the Chinese medicine into the process of modernization,so exploring the active component of traditional Chinese medicine has become the point of the entire pharmaceutical research.Chinese medicine is widely applied in the cure of many diseases and women's health.Phytoestrogens in them account for their pharmacological effects.Cistanche deserticola is known as "desert ginseng",considereing to have nourishing,strong effect.The issue through the whole animal experiments(animals Uterotrophic France)and in vitro cell proliferation assay to screen its phytoestrogen antagonism efficacy parts.The experiment uses HPLC to characterization the influences on different processing methods of drug-containing serums,urine,and feces of rats,and establish the liquid chromatogram of biological samples,and analyze the chemical composition and structure identification with mass spectrometry.It is significant of discovering the source of estrogen antagonist effective in new medicine from the Chinese medicine and the use of clinical disease.(1)Using the whole animal experiments(animal uterine weight method)to value the changes of uterus factor and after taking drug,evaluate the estrogen effects of the test substance and its medicated serum on MCF-7 cell proliferation by MTT assay.The results show that 95%ethanol extract parts of 40g-kg-1·d-1 can significantly inhibit uterine growth caused by positive drug(P<0.01),and its medicated serum can significantly inhibit MCF-7 proliferation test(P<0.01).The comprehensive results show that 95%ethanol extract parts of 40 g-kg-1·d-1 is the best active part,and dosage of gavage.(2)The experiment uses HPLC-Q-TOF/MS,26 chromatographic peaks were marked in the Cistanche deserticola.Compared with standard substance and MS information,24 chemical constituents were identified;Kankanoside H1?Geniposidic acid,Decaffeoylacteoside,8-epiloganic acid,Salidroside,Syringalide-A3'-?-rhamnopyranoside,Cistanoside A,Echinacoside,Cistanoside F,Cistanoside B,Tubuloside A,Osmanthuside B,Cistanoside C,Acteoside,Isoacteoside,Cistanoside D,Tubuloside B,2'-Acetylacteoside,Cistanoside G,Kankanoside E,Campneoside,Cis-acteoside,Campneoside,Isoacteoside,Cistantubuloside B1.(3)The experiment uses HPLC-Q-TOF/MS,12 chromatographic peaks were marked in the rat serum under the anti-estrogen effective dose.Compared with standard substance and MS information,12 chemical constituents were identified:containing 6 prototype constituents:Geniposidic acid,Echinacoside,Acteoside,Cistanoside A,Cistanoside D,Salidroside;and 6 metabolites:3,4-dihydroxyphenethyl glycoside,3,4-dihydroxyphenethy,Cistanoside B glucur-onide conjugation,Methyl 3,4-dihydroxyphenethyl glycoside,3,4-dihydroxyphenethyl sulphate conjugation,Acteoside sulphate conjugation.(4)This part,the experiment uses the same methods methods to mark 15 chemical constituents in rat urine,containing 7 prototype constituents:Geniposidic acid,Salidroside,Syringalide A3'-?-rhamnopyranoside,Echinacoside,2'-Acetylacteoside,kankanoside H1,Acteoside and 8 metabolites:Cistanoside G sulfate conjugation?Methyl 8-epiloganic acid,Hydroxyl methyl 3,4-dihydroxyphenethyl glycoside,Homovanillic alcohol glucuronide conjugation,Methyl caffeic acid sulfate conjugation,Salidroside glutamic acid metabolite,Hydroxytyrosol glucuronide conjugation,Caffeic acid glucuronide conjugation.(5)This part,the experiment uses the same methods methods to mark 15 chemical constituents in rat feces,containing 7 prototype constituents:Cistanoside D,Salidroside,Decaffeoylacteoside,Cistanoside G,Tubuloside A,Echinacoside,Acteoside and 8 metabolites:3,4-dihydroxyphenethyl alcohol,Cistanoside B sulfate conjugation,Lugrandoside,Acetyl Cistanoside F,Acetyl Cistanoside D,Methyl 8-Epiloganic acide,Caffeic acid,Hydroxyl methyl 3,4-dihydroxy phenethyl glycoside.This experiment illustrates more comprehensively the material base of Cistanche deserticola in phytoestrogen antagonism effect.Reveals the 95%ethanol extract of 40 dose group is the effective part.Using HPLC-Q-TOF/MS technology to analyze and identify the Cistanche deserticola in biological samples,lays a basis on the further study of Cistanche deserticola chemical composition.