Expression Of PGP9.5?GFAP And GDNF In Intestinal Injury Induced With NSAIDs In Rats:Research On Protective Effects Of Berberine

Background NSAIDs are widely used,however,these drugs can have both beneficial and harmful effects on athletes.A large number of studies have confirmed that it can make lower gastrointestinal tract hurt more than upper gastrointestinal tract.NSAIDs gastrointestinal mucosal injury is a common adverse reaction.The enteric nervous system(ENS)is one of the major regulator for the intestinal mucosal barrier.In the research,the EGCs and its active substance play an important role in maintaining the integrity of the intestinal mucosal barrier.Therefore,this paper through the establishment of NSAIDs enteropathy rat model to observe the effect of berberine on NSAIDs enteropathy in the enteric nervous system,as a result,berberine will be a new drug for an effective way to treat NSAIDs related intestinal injury.Objective To explore the protective effect and influence on the expression of GDNF?PGP9.5(a neuronal marker in the enter nervous system)?GFAP(EGCs' marker)of berberine on NSAIDs(Non-steroidal anti-inflammatory drugs)related intestinal injury.Method 40 males SD rats were divided into five groups randonly:control group,model group,receiving diclofenac sodium 7.5mg/(kg d)for 5 days;and three protection groups,receiving berberine 25mg/(kg d),50mg/(kg d),75mg/(kg d)for 3days,respectively,beginning after the sixth day given the diclofenac sodium.hntestinal mucosa were taken 24 hours after last drug administration to observe the general,histological injuries and PGP9.5,GFAP,GDNF expression.Result The histological score and the general score in model control group are respectively 5.75 and 4.83.Scores in 50mg/(kg d)?75mg/(kg d)berberine protection group is lower than in model group(P<0.05);PGP9.5?GFAP?GDNF content in normal control group and high dose protection group are significantly higher than the other groups(P<0.05);PGP9.5?GFAP?GDNF content in model group and in each dose of berberine group expression is significantly lower than the one in control groups(P<0.05),PGP9.5?GFAP?GDNF content in control group and in other protection group is higher than in model groups(P<0.05).Conclusion Berberine can exert protective effect on intestinal injury induced by non-steroidal anti-inflammatory drugs and its mechanism is related to the increase of PGP9.5,GFAP and GDNF.