Study On Main Chemical Components And Anti-inflammation Activity Of Euscaphis Konishii Hayata’s Seeds And Pericarps

Euscaphis konishii Hayata,which is the plant of Staphyleaceae Euscaphis,As a traditional medicine in China,its seeds,pericarps,leaves,barks and flowers and other parts can be used as medicine for dysentery,diarrhea,colic,metrorrhagia,rheumatism,traumatic injury,swelling and pain,stomach pain,hernia,treatment of irregular menstruation and so on.In this paper,by using different physicochemical experiment,the main active constituents of Euscaphis konishii Hayata’s seeds and pericarps were qualitativiy detected.The water extract and alcohol extract of Euscaphis konishii Hayata’s seeds and pericarps and its extraction part were tested in anti-inflammatory and capillary permeability of pharmacological experiments.The part of n-butyl alcohol extraction of water extract of Euscaphis konishii Hayata’s pericarps was separated and purified by macroporous adsorption resin,silica gel column chromatography chromatography and MPLC,and two compounds were separated,and they were tested purity by HPLC.The two compounds’ structure were analysised and indentified by IR,MS,1H-NHR and 13C-NHR.The main results are as follows.Firstly,Euscaphis konishii Hayata’s seeds contain saponins,triterpenes,sterides,flavonoids and its glycosides,oil,coumarin,lactone and polysaccharides and their glycosides,and that Euscaphis konishii Hayata’s pericarps contain saponins,phenolic compounds and tannins,triterpenes,flavonoids and its glycosides,anthraquinones and polysaccharides and their glycosides.Secondly,in the anti-inflammatory experiments show that the water extraction and the n-butanol fraction of pericarps are about 5%higher than the positive control in anti-inflammatory activity,in the more than 65%;In the capillary permeability of pharmacological experiments show that the water extraction and the n-butanol fraction of pericarps are about 5%higher than the positive control in anti-inflammatory activity,in the more than 60%.Thirdiy,the part of n-butyl alcohol extraction of water extract of Euscaphis konishii Hayata’s pericarps was separated and purified,and two compounds were separated,and they were tested purity by HPLC,and the purity was more than 95%.The two compounds’structure were analysised and indentified,and it is likely to be Astragaloside A and Isoquercitrin.Astragaloside A is the derivatives of tetracyclic triterpenoid saponins,has good effect of sedation and analgesia,anti-inflammatory,antihypertensive antiviral,enhance immunity,improve heart and lung function.Isoquercitrin is the flavonoid glycoside derivatives,has antibacterial,antihypertensive,anti-inflammatory activity.