The Detecting Study Of Cefoperazone Sodium-sulbactam Sodium And Biotin Based On Room Temperature Phosphorescence Quantum Dots

Developing and exploring a high sensitivity analysis and detection method for bioactive molecules and drug molecules that existed in biological and biological processes,is a hot and difficult issue in the field of modern biological technology.And semiconductor nano-quantum dots have been widely concerned in the field of biochemical analysis and medical research with its excellent optical properties and easily surface modification.It has been found that doping of simple quantum dots can make the photo-excitation efficiency higher and can produce stronger phosphorescence.When in the process of detection,the room temperature phosphorescence(RTP)nano-quantum dots can effectively avoid interference from biological fluid background fluorescence and scattered light,without adding any deoxidizer and inducer,making it more suitable for the detection and identification of target molecules in biological samples.Based on the above considerations,this study aimed at making full use of the advantages of related disciplines,through the surface modification of room temperature phosphorescence quantum dots,then combining the nanotechnology with optical sensor technology to construct several nano-sensors,which were used for the trace detecting application of molecules and biomolecules.The main research contents are as follows:1.3-mercaptopropionic acid-capped Mn-doped Zn S quantum dots(MPA-Mn:Zn S QDs)were synthesized in the aqueous phase and characterized by transmission electron microscopy,X-ray diffraction electron microscopy,fluorescence spectroscopy and phosphorescence spectroscopy.Based on the phosphorescence quenching effect of cefoperazone sodium-sulbactam sodium(CPZ-SBT)on quantum dots,a method for trace detection of cefoperazone sodium-sulbactam sodium is established.The detection based on the3phenomenon that the electron acceptor acted by CPZ-SBT can effectively quench the effect of RTP of MPA-Mn:Zn S QDs through electron transfer.There is a good relationship between CPZ-SBT and changes on the phosphorescence intensity of MPA-Mn:Zn S QDs when the concentration of CPZ-SBT is in the scope of 0.7~84 ?g/L.The correlation coefficient and detection limit of this method is 0.99 and 0.14 ?g/L respectively.The method is simple to analyze,without deoxidizer,inducer and complex sample treatment.2.A switchable room-temperature phosphorescence(RTP)nanosensor based on an MPA-capped Mn-doped Zn S QDs/CTAB composite system was established for the detection of biotin.The phosphorescence intensity of QDs/CTAB could be regularly quenched with the increasing of biotin.Under optimal conditions,this method yielded two linear ranges of 2?g/L to 20 ?g/L and 20 ?g/L to 140 ?g/L with respective correlation coefficients of 0.993 and0.990 and a detection limit of 0.93 ?g/L.Therefore,the analytical potential of the proposed nanosensor was evaluated by detection of biotin in urine and biotin tablets.This approach yielded satisfactory results because of the effective elimination of background fluorescence and light scattering from the sample matrix.This approach provides a practical method for biotin detection.In short,we synthesized MPA-capped Mn-doped Zn S quantum dots with excellent luminous properties by simple aqueous phase method.Subsequently,we established trace detection method of cefoperazone sodium sulbactam sodium and biotin.The method shows a lower detection limit and wider linear range,and can effectively avoid the fluorescence interference from background.The method provides a new method for the detection of cefoperazone sodium sulbactam sodium and biotin,with advantages of simple operation,excellent selectivity and stability.