Study On The Detection Of Small Drug Molecules By Room Temperature Phosphorescence Quantum Dots

Quantum dots,as a kind of excellent nanomaterials,possess a large number of advantages of simple preparation method,adjustable size and easy surface modification.Therefore,it was studied and applied so widely in analytical chemistry and biomedical fields that becoming a hot topic in current scientific research.However,the doped quantum dots hold better performance than the pure quantum dots because of the introduction of new elements,thus jump to material star.However,the research on doping quantum dots mostly focuses on its fluorescence properties,and there is still little research on its phosphorescence properties.Phosphorescence,with longer lifetime than fluorescence,allows an appropriate delay time and can be free from the interferences from autofluorescence and scattering light.In addition,the selectivity of room temperature phosphorescence detection can be further improved owing to its rarely seen than fluorescence;Moreover,it need no more complex sample pretreatment,which greatly simplifying the target detection process.This provides a new opportunity for material analysis and detection based on quantum dots room temperature phosphorescence.Therefore,this paper,which based on the combination between superior phosphorescence properties of 3-mercaptopropionic acid(MPA)coated Mn-doped Zn S quantum dots(MPA-Mn:Zn S QDs)and chemical structure characteristics of target analyte,is aimed at constructing a room temperature phosphorescence(RTP)analysis system of three substances,such as diprophylline,artemisinin and resveratrol.The full text is divided into four part,the main contents are as follows:1.Based on the aqueous phase synthesis method,3-mercaptopropionic acid(MPA)coated Mn-doped Zn S quantum dots(MPA-Mn:Zn S QDs)were prepared and characterized.Then,with the room temperature phosphorescence quenching effect of MPA-Mn:Zn S QDs by diprophylline,a simple and rapid method for the detection of diprophylline was developed.The linear range of the method is 1.97~197 n M,the correlation coefficient is 0.998,and the3 detection limit is 0.893 n M.The detection was mainly based on the positively charged diprophylline adsorbed on the surface of Mn-doped Zn S quantum dots by electrostatic interaction and further quenched the room temperature phosphorescence of Mn-doped Zn S quantum dots by electron transfer effect.The method needs no complex pre-treatment of actual sample when detecting diprophylline in it,which was demonstrated with excellent analytical performance.2.Based on the quenching effect of artemisinin on the phosphorescence of3-mercaptopropionic acid coated Mn-doped Zn S quantum dots(MPA-Mn:Zn S QDs),a phosphorescence method for artemisinin detecion was established.There was a good linear relationship between the concentration of artemisinin and the luminescent intensity of quantum dots in the range of 0~1.77 n M and 1.77~35 n M,the detection limit was 0.18 n M.The method is successfully applied to the detection of artemisinin in the actual sample and the process is simple.In addition,because the phosphorescence is more rarely than fluorescence,the selectivity of detection can be further strengthened.3.Based on the regular quenching effect of resveratrol on the RTP of Mn-doped Zn S quantum dots,a phosphorescence quenching method for the detection of resveratrol in biological fluids and actual drugs was established.The process mainly based on the formation of ground complex through hydrogen bonding interactions between polyhydroxy resveratrol and MPA in surface of quantum dots under the conditions of p H 6.4.Subsequently,the ground complex is resulted in the enhancing efficiency of electron non-radiation,which further decreased the phosphorescence intensity of quantum dots.Under the optimal conditions,the present assay was allowed for detecting resveratrol in the range of 0.03 ?M to 14 ?M with a detection limit of 0.01 ?M,and this method was successfully applied to the detecting resveratrol in actual samples.The performance of this method is excellent.In summary,the detection methods of three common drugs were established based on the excellent room-temperature phosphorescence properties of MPA-capped Mn-doped Zn S QDs.The addition of the target could resulted in regularly change of QDs phosphorescence.Besides,the mechanism of every system was also explored.As it should be,the established system has overcome the shortcomings of existing methods,and solved the simple and efficient drug testing problems in current stage.Futhermore,it also provides a convenient,low-cost and highly effective alternative method for clinical detection of related drugs.