Effects Of Doxorubicin-loading Mesoporous Silica Nanoparticles On Human Tongue Cancer Cells

Objective: To investigate the effects of doxorubicin-loading mesoporous silica nanoparticles(MSNs@DOX)on the proliferation and apoptosis of human tongue cancer cells(Tca-8113)in vitro,and explore the possible mechanisms,preliminarily.Methods: MSNs were characterized by transmission electron microscope(TEM)and N2 adsorption/ desorption method.The drug release profiles of DOX from MSNs were monitored through UV-Vis.The biological safety of MSNs was determined by MTT colorimetry.The anticancer effect of MSNs@DOX on Tca-8113 cells was demonstrated by MTT assay,optical microscope,Hoechst 33342 staining and flow cytometry,and the possible mechanism was explored by Western blot.Result:(1)MSNs had a spherical shape and uniform distribution with a diameter of about 30 nm.Its average pore size,suface area and pore volume were 19.59682 nm,193.4296 m2/g and 0.947625 cm3/g,respectively.With the increase of concentration of MSNs,the release time of DOX extended gradually,and the accumulative release rate increased.(2)MSNs displayed a good cytocompatibility.MSNs@DOX inhibited the proliferation of Tca-8113 cells in a dose-time dependent manner(P<0.05).(3)With the increase of concentration of MSNs,the apoptosis of Tca-8113 cells was induced by MSNs@DOX remarkably and the cell cycle was arrested at G0/G1 checkpoints(P<0.05).(4)With the increase of concentration of MSNs,the expression of Bax,p53 and caspase-3 of Tca-8113 cells was up-regulated and that of Bcl-2 down-regulated,gradually(P<0.05).Conclusion: MSNs@DOX could inhibit the proliferation and induce the apoptosis of Tca-8113 cells in a dose-time dependent manner,and the mechanisms may be related to regulation of cell cycle and expression of proteins in cells.