Preliminary Study Of Petroleum Ether Extract Of Lamiophlomis Rotate On Migration,Adhesion And Invasion Of Tca8113 Cell Line

Objective: Preparation of petroleum ether extract Lamiophlomis Rotate(PEELR)and discussion of its effect on migration,adhesion and invasion of human tongue squamous cell carcinoma Tca8113 cells.Materials and Methods: In present study,we can prepare the PPELR.The human tongue squamous cell carcinoma cell line Tca8113 as the research object,the experiments are divided into three groups: PEELR treatment group,control group,positive control group.MTT method was used to study the effect of PEELR on proliferation of Tca8113 cells,the scratch test and Transwell chamber migration assay was used to study the effect of PEELR on Tca8113 cell migration.Transwell chamber invasion assay was used to detect PEELR on the invasion of Tca8113.cell matrix adhesion assay was used to study the PEELR effect on Tca8113 cell adhesion,and finally using Western blot experiments to test PEELR on MMP-2,MMP-9,STAT-3,VEGF protein expression.Results: 1.24 h MTT showed that 24 h IC5053.83?g/mL with increasing drug concentration and prolonging the time,the inhibition rate of cell growth are increased Regardless of the PELLR drug group or the positive control group,the difference in cell proliferation inhibition was statistically significant(P<0.05)compared with that in the blank control group.2.Tca8113 cells have strong ability of migration,with the increasing of PEELR concentration,scratch width was increasing,Regardless of the PELLR drug group or the positive control group,the difference in cell migration inhibition was statistically significant(P<0.05)compared with that in the blank control group.3.After 24 hours,Tca8113 cells through the basement membrane to the bottom side,with the increasing of drug concentration,the number of cells was decreasing which through the chamber.Regardless of the PELLR drug group or the positive control group,the difference in cell migration inhibition was statistically significant(P<0.05)compared with that in the blank control group.4.With the PEELR acting on cells 24 hours,Tca8113 cell adhesed to the basement membrane.When PEELR concentration was increasing,the difference in cell adhesion inhibition was statistically significant(P<0.05)compared with that in the blank control group.5.After 24 hours,the Matrigel matrix was decomposed by Tca8113 cells,and the number of passes through the membrane was decreased.With the concentration increasing,the invasion cell number was decreased,the difference in cell migration inhibition was statistically significant compared with that in the blank control group and PELLR group(P<0.05).6.The protein expression of MMP-2,MMP-9,STAT-3 and VEGF was decreased with the increasing of PEELR concentration.The concentrations of MMP-2,MMP-9,STAT-3 and VEGF could be reduced by PEELR in each concentration group.Compared with the blank control group,the difference of the protein expression between the PEELR drug group and the blank control group was significant(P<0.05).Conclusion: 1.PEELR can inhibit the migration,adhesion and invasion of Tca8113 cells.2.PEELE inhibits the migration,adhesion,and invasion of Tca8113 cells and may be achieved by lowering the protein expression of MMP-2,MMP-9,STAT-3 and VEGF.