Macrophage Immune Regulation Research Of Uygur Medicine Brassica Rapa L Polysaccharide

Objective: This subject on the basis of pre-scholars extract on Brassica rapa L polysaccharide Systematically macrophages are the immune regulation of experimental research,in the hope of the people of ancient xinjiang Chinese drugs just for the future development provides the basis for clinical application.Methods:1.Determination of water content of medicinal materials,and 1 will just Brassica rapa L after crushing,adopt continuous reflux heating method,material liquid than 25 water 3 h,2 times,to merge the filtrate after enrichment,after adding ethanol alcohol content of 80%,will just Brassica rapa L polysaccharide low-temperature vacuum drying to constant weight,weighing,using the right amount of distilled water configured to 5 mg/ml of polysaccharide reserve liquid,add triploid polysaccharide product in reserve liquid chloroform: n-butyl alcohol mixture((V: V)= 3:1),repeated oscillation in addition to protein,and water heating and 60 ℃,join the volume 5% activated carbon,let stand for 30 min in 60 ℃ oven,cloth’s suction filter funnel,filtrate the evaporating dish in water bath pot enrichment and alcohol sink the alcohol content of 80%,after 4 ℃ refrigerator let stand for the night,precipitation temperature drying to constant weight,both to d medicine just to Brassica rapa L polysaccharide,mapping table to glucose to determine the content of polysaccharide,and calculate the yield.2.The antioxidant effect was studied by using BRLP and powder tablets to remove free radical experiments.3.Cultivate macrophage cell immune regulation and do a series of experiments,just Brassica rapa L polysaccharide on macrophage proliferation activity and the active role of macrophage cell and macrophage NO effect in the research,research on the effects of macrophages to secrete TNF alpha,and western blot method to detect just ancient polysaccharide on the macrophage proteins characteristic,the influence of the expression and distribution.4.To study the molding process of BRLP tablets.Result:1.To glucose levels do the abscissa,ordinate absorbance do draw the glucose standard curve for Y = 55.04 + 0.0096 X(r2 = 0.9994)d medicine just Brassica rapa L polysaccharide content in 38.27%,just Brassica rapa L polysaccharide yield reached 35.16%.2.Uygur medicine jus Brassica rapa L polysaccharide on DPPH free radical and hydroxyl free radical has obvious removal ability,show that just Brassica rapa L polysaccharide extract has the ability of scavenging free radicals and antioxidant activity,and found in the test just Brassica rapa L polysaccharide’s ability to remove free radicals have clear concentration-response relationship.3.Just Brassica rapa L polysaccharide can promote proliferation activity,macrophage activity,promote the secretion of macrophage NO and TNF alpha.4.It is determined that 80% of the ethanol is adhesives,the lactose as the diluent,the 5% of CMS-Na as the disintegrants and the magnesium stearate as the lubricants is the best Molding process.Conclusion: Uygur medicine Brassica rapa L polysaccharide has good macrophages can enhance antitumor,antivirus,inflammation and immune response ability,enhance immunity effect.