The Preparation Of Multilayer Heart Valve Scaffold Based On Poly(Ethylene Glycol) Diacrylate And Collagen Fiber

American Heart Association reported,that in the United States alone,heart valve disease was the sole cause of mortality in 15,257 deaths.Thus,there is crunch time that treat of heart valve disease.The most common treatment for valvular disease is implantation of a prosthetic valve.Several researchers are therefore exploring tissue engineering strategies towards the development of a heart valve equivalent.In this paper,Tissue engineering scaffolds were constructed by Polyethylene glycol diacrylate(PEGDA)with better biocompatible and egg membrane(ESM)with better mechanical properties.On the one hand,it can solve the biocompatibility problem.On the other hand,it also can solve the problem of mechanical properties.The main results of this experiment are as follows:(1)Use cold light source light method,preparation of PEGDA hydrogel from PEGDA,and study the swelling rate,porosity and mechanical properties of the hydrogel.Experimental results show that PEGDA swelling rate is higher,the swelling ratio was inversely proportional to the concentration of PEGDA hydrogel;PEGDA hydrogel without GA cross linking,concentrations rise from 15%to 30%,Porosity rise from(52.36±2.48)%to(59.72±3.91)%,with glutaraldehyde crosslinking,Porosity rise from(61.28±3.11)%to(70.16±3.71)%;The concentration of 15%,20%,30%PEGDA hydrogel,the highest modulus of elasticity was not more than 0.20Mpa,and according to the literature,the elastic modulus of the primary tissue circumferential elastic modulus can reach 5.87Mpa,radial elastic modulus can reach 1Mpa.(2)Compound PEGDA hydrogel and ESM to preparate scaffolds and study the swelling rate,porosity and mechanical properties of the hydrogel.Experimental results show that The circumferential and radial elastic moduli of the scaffolds were(4.67±0.05)MPa and(2.48±0.13)Mpa,after crosslinking with glutaraldehyde(GA),the circumferential and radial elastic moduli were(10.41±0.33)MPa and(6.30±0.16)Mpa;PEGDA concentration for 15%,20%,30%of the scaffold's swelling rate are low,scaffold soaked in pure water 1 week(1W),the swelling rate(0.42?0.2)%,soak for two weeks(2W),the swelling rate for(0.34?0.25)%,therefore the swelling rate is negligible;Scaffolds without GA cross linking,PEGDA concentrations rise from 15%to 30%,porosity rise from(62.02±1.41)%to(69.63±2.44)%,with glutaraldehyde crosslinking,porosity rise from(71.28±3.01)%to(80.16±3.88)%,it can provide a lot of space for cell proliferation;The results showed that the enzymatic hydrolysis of the original scaffold and GA scaffold was negligible,and the mechanical characterization experiments showed that the mechanical properties of the scaffolds were not affected by enzymatic hydrolysis.(3)The scaffold for simple animal experiments.Experimental results suggest after the scaffold was implanted in mice,the tissue that was close to the scaffold did not produce lesions and inflammation.Therefore,the mice had good biocompatibility with the mice.