ACE2 Prevents Endothelial Dysfunction,Insulin Resistance And Hepatic Fibrosis In ApoE-/- Mice Fed With High-Fat Diet

Objective: To investigate the effects of transfection of recombinant adenovirus vector carrying ACE2 gene(Ad-ACE2)on the protection of endothelium,insulin resistance and hepatic fibrosis in high-fat-fed ApoE-/-mice.Methods:Thirty ApoE-/-mice were randomly divided into control group(Con group),empty vector group(EGFP group)and ACE2 gene therapy group(ACE2 group).They Were all given high-fat diet,the control group were injected through the tail vein injection of saline,the EGFP group were injected with Ad-EGFP and the ACE2 group were injected with Ad-ACE2,glucose tolerance and insulin resistance at 12 weeks of the experiment.Take blood to determine the level of blood lipids,the changes of ACE2,COL-?,COL-?,and IL-6 were observed by HE staining,oil red O staining and immunohistochemistry.The expression of ACE2 in liver was detected by Western Blot.Results: The ACE2 gene transfection with Ad-ACE2 significantly increased the expression of ACE2 in the liver of ApoE-/-mice(P < 0.05).The endothelium-dependent function of ACE2-treated group was significantly better than that of Con group and EGFP group(P<0.05).The insulin sensitivity was stronger than that of Con group and EGFP group(P<0.05),and lipid levels did not change significantly.The expression of COL-?,COL-?,and IL-6 in the liver tissue of ACE2 treatment group were lower than those in Con group and EGFP group(P<0.05).Conclusion:ACE2 overexpression can protect the endothelium,improve insulin resistance,reduce inflammation,inhibit liver fibrosis and thus protect liver function.