The Construction Of Carbon Dots-Gold Nanoparticles Complex And Its Chiral Response

Objective In this study,we pointed out a novel strategy for the construction of C-dots@Au NPs complex,established a simple and rapid chiral response platform,which free from any surface modification of nanoparticles,to realize the chiral recognition of glucose isomers with colorimetric and fluorescence dual modes.Methods H₂O₂ was used as a reductant to reduce metal salts produce metal nanoparticles,further mediated the self-formation of carbon dots-gold nanoparticles complex?C-dots@Au NPs complex?.At the same time,combined with stereospecificity of glucose oxidase?Gox?,which can specifically catalyze D-glucose to generate H₂O₂,and then utilized this chiral response event to mediate the formation of C-dots@Au NPs complex.First,a sulfur doped C-dots has been synthesized by a one-pot hydrothermal carbonization of citric acid with cysteine?Cys?as the sulfur source,which provided a driven power of Au-S interaction for the formation of C-dots@Au NPs complex.Then,in the present of sulfur doped C-dots and gold salts mixture solution,H₂O₂ reduced AuCl₄^-to generate Au NPs in situ,which promoted the formation of C-dots@Au NPs complex fast.Owing to the nanocomplex acquired not only had unique surface plasmon resonance?SPR?property,but also displayed fluorescence quenching effect of C-dots based on a fluorescence resonance energy transfer?FRET?phenomenon,this complex could be used to quantities analysis for H₂O₂ by colorimetric and fluorescence modes.Second,glucose oxidase can especially catalyze D-glucose to generate H₂O₂,further H₂O₂ induced the construction of C-dots@Au NPs complex,through the different changes of colorimetric and fluorescence signals,to realize the dual-mode distinguish of glucose enantiomers.Results A sulfur doped C-dots acquired in this study with uniform size,good dispersion and fluorescence quantum dots?QY?was up to 37.6%.Further,we successfully constructed a C-dots@Au NPs complex using H₂O₂ as reductant,which had colorimetric and fluorescence dual response to H₂O₂,and the limitation of response was 0.12?M and 0.89?M?3?/S?,respectively.Similarly,an enzymatic reaction system with D-glucose as substrate had H₂O₂ generated,it further mediated the formation of C-dots@Au NPs complex,which had colorimetric and fluorescence dual response to D-/L-glucose.Conclusion A construction method of C-dots@Au NPs complex induced by H₂O₂ has been pointed out,and the nanocomplex acquired had colorimetric and fluorescence dual-mode response toward H₂O₂.A chiral response platform based on C-dots@Au NPs complex mediated by enzymatic reaction system was successfully constructed,and was successfully used for the bimodal recognition of glucose enantiomers.The platform didn't need any modification strategies of nanoparticles,which had synthesis simple,response fast,high efficiency and universality.