Applications On Both Quantum Dots And Gold Nanoparticles For Pharmaceutical Detection

The fluorescence and colorimetric visualization assay has been increasingly employed due to its simplicity, selectivity, and cost-effective nature and no requirement of sophisticated instruments. The probe method can increase its sensitivity and selectivity. In this paper, two novel probes for the determination of pharmaceuticals have been developed. The first method was based on the fluorescence of thioglycolic acid-capped CdTe quantum dots in aqueous solutions. The fluorescence of CdTe QDs can be enhancement or quenched by the different pharmaceuticals. The second method was a simple colorimetric method for trace pharmaceuticals with the unmodified gold nanoparticles as the sensing probe. Multiple N-donor functional groups of pharmaceuticals molecules has a the high binding affinity to AuNPs. This is presumed to be able to directly cause the aggregation of AuNPs. The main results are outlined as followings:1. The enhancement of the fluorescence of CdTe QDs and the aggregation of unmodified AuNPs by Kanamycin sulfate in aqueous solutions have been developed. Under optimum conditions, the relative fluorescence intensity was linearly proportional to the concentration of Kan between0.04μg mL-1to0.80μg mL-1. The linear ranges of the colorimetric sensor for kanamycin sulfate were from3.810-3μg mL-1to4.110-2μg mL-1and from4.910-2μg mL-1to8.610-2μg mL-1, respectively. Comparison between QDs and Gold nanoparticles were made, the recoveries of the two methods were in the range of96.92%to104.01%. Both the two methods were used for rapid detection of Kan in injections with satisfactory results.2. The enhancement of the fluorescence of CdTe QDs and the aggregation of unmodified AuNPs by streptomycin sulphate(Str) in aqueous solutions have been developed. Under optimum conditions, the relative fluorescence intensity was linearly proportional to the concentration of Str between0.16μg mL-1to9.6μg mL-1. The linear range of the colorimetric sensor for Str was from0.11μg mL-1to3.18μg mL-1. Comparisons between the two methods were made, the recoveries of the two methods were in the range of97.5%to103.75%. Both methods were used for rapid detection of Str in the tablets with satisfactory results.3. Two novel methods are used for the determination of FNC (4’-azido-2’-deoxyfluoroarabinocytidine) has been developed based on enhancement of the fluorescence of CdTe QDs and the aggregation of unmodified AuNPs in aqueous solutions. Under optimum conditions, the relative fluorescence intensity was linearly proportional to the concentration of FNC between0.65μg mL-1to77.47μg mL-1. The linear range of the colorimetric sensor for FNC was from6.9×10-2μg mL-1to9.0×10-1μg mL-1. Comparisons between the two methods were made, the recoveries of the two methods were in the range of95.07%to104.67%. Both methods were used for rapid detection of FNC in synthetic samples with satisfactory results.4. Two novel methods are used for the determination of6-fluoro-9H-pyrrolo [1,2-a]indol-9-one (hereinafter referred to as the FPIO) has been developed based on quenching of the fluorescence of CdTe QDs and the aggregation of unmodified AuNPs in aqueous solutions. FPIO is a fluoride derivative of9H-pyrrolo[1,2-a] indol-9-one,which show a broad spectrum of biological activities including antidiabetic, psychostimulant and anticancer. Under optimum conditions, the relative fluorescence intensity was linearly proportional to the concentration of FPIO between0.12μg mL-1to12.47μg mL-1. The linear ranges of the colorimetric sensor for FPIO were from7.5×10-2μg mL-1to23.94μg mL-1, respectively. Comparison between QDs and Gold nanoparticles were made, the recoveries of the two methods were in the range of96.46%to104.57%. Both the two methods were used for rapid detection of FPIO with satisfactory results.