| OBJECTIVE:To investigate the relationship between the CTC counts in peripheral blood and the clinic features and prognosis in patients after radical resection of stage ?-? colorectal cancer.To evaluate the association between CTC counts and clinic features and therapeutic response in the patients with advanced colorectal cancer.METHODS:Between October 1,2014 and December 31,2016,115 colorectal cancer patients who were diagnosed and treated at Zhongshan Hospital Affiliated to Dalian University were enrolled in this study,including 63 patients after radical resection of stage ?-? colorectal cancer and 52 patients with advanced colorectal cancer.115 patients were collected with 7.5ml peripheral blood to detect and analysis of CTC enumeration by Cell Search system.CTC?1 was determined as positive cutoff value.According to statistics,63 patients with stage ?-? colorectal cancer received blood collection after radical operation and 52 patients with advanced colorectal cancer received blood chemotherapy during chemotherapy.Analyze the relationship between CTC counts and the clinical characteristics and DFS of 63 patients after radical resection of stage ?-? colorectal cancer.Evaluate the relationship between CTC counts and the clinical characteristics,therapeutic response and PFS of 52 patients with advanced colorectal cancer.RESULTS:The CTC value of 63 patients with stage ?-? colorectal cancer was(0.57± 1.51)/7.5ml,and the positive rate was 19.04%(12/63).The CTC counts were not related to age,sex,tumor position,TNM staging,CA199 and CEA in patients with stage ?-? radical resection of colorectal cancer(P>0.05).The median disease-free survival time of CTC negative group was significantly higher than that of CTC positive group after radical resection of stage ?-?,but the difference was not statistically significant(P=0.560).The CTC value of 52 patients with advanced first-line chemotherapy for colorectal cancer was(3.52±7.69)/7.5ml,and the positive rate was 46.15%(24/52).The CTC counts in patients with advanced first-line chemotherapy were not related to age,sex,tumor location,metastasis site,CA199 and CEA(P>0.05).Of the 52 patients with advanced colorectal cancer,positive and negative CTC were 24 cases(46.15%)and 28 cases(53.85%).CTC counts in 52 patients with advanced colorectal cancer were not correlated with patient,s clinical characteristics(P>0.05).52 cases of advanced first-line chemotherapy of colorectal cancer were divided into 3 groups according to the time of collecting CTC.28 cases,10 cases and 14 cases were detected at baseline state and after the 2 and the other cycles respectively.The positive rate of CTC in each group was significantly higher than that in non-progressive patients.And the median PFS in each group of CTC negative groups was significantly higher than that of the CTC positive group,and the difference was statistically significant(P=0.027,P=0.022,P=0.003).52 cases of advanced first-line chemotherapy of colorectal cancer were divided into 3 groups according to the different chemotherapy regimens.22 cases,16 cases and 14 cases were treated with FOLFOX regimens,FOLFIRI regimens and other regimens respectively.The positive rate of CTC in each group was significantly higher than that in non-progressive patients.The median PFS in CTC negative group was significantly higher than that in CTC positive group during each chemotherapy period,the difference was statistically significant(P=0.001,P=0.009,P=0.034).There was no correlation between serum tumor markers CEA and CA199 in 52 patients with advanced colorectal cancer and median PFS in patients(P>0.05).CONCLUSIONS:CTC counts in patients after radical resection of stage ?-? colorectal cancer and advanced patients were not related with clinical characteristics.Patients with negative CTC counts in peripheral blood have a longer DFS after radical resection of stage ?-?.CTC counts in advanced patients was related with therapeutic response and PFS which needs further evidence with larger number of clinical data. |
PDF Full Text Request