The Expression And Significance Of IL-31、IL-33 And Its Receptor ST2 In Refractory Rhinosinusitis

Objective: interleukinIL-31(IL-31)and interleukin-33(IL-33)plays an important role in many inflammatory and autoimmune diseases.The purpose of this study was to investigate the expression of IL-31,IL-33 and its receptor ST2,eosinophilia eosinophilus,and total IgE in peripheral blood of patients with refractory rhinosinusitis(RRS).To investigate their role in the pathogenesis of RRS,and the possible effect of allergic factors on refractory sinusitis.Methods: Case screening,the experimental group,drawn specimens and experimental methods.The selected cases were hospitalized patients from February 2017 to July 2017 period Otolaryngology Head and Neck Surgery at the First Affiliated Hospital of Guangxi Medical University,receiving functional endoscopic sinus surgery routinely.In this study,a refractory group,an initial treatment group,and a control group were established.The hematoxylin and eosin staining method was used to observe the morphology and detect the number of eosinophils(EOS)present in the mucosal tissues.The relative levels of IL-31,IL-33,and ST2 in the tissues were detected by immunohistological examination.Quantitative fluorescence real-time polymerase chain reaction wasused to detect the mRNA levels of IL-31,IL-33,and ST2,and total serum immunoglobulin E(IgE)expression in the peripheral blood.The experimental data were statistically analyzed by SPSS 22.0 software.Results:(1)The number of EOS in the refractory group and initial treatment group were higher than that in the control group.Further,the number of EOS in the refractory group were higher than that in the initial group.(2)On immunohistological examination,the expression levels of IL-31 and IL-33 proteins in the refractory group and the initial treatment group were higher than those in the control group.Additionally,the expression levels of both IL-31 and IL-33 were higher in the refractory group than in the initial treatment group.The expression of ST2 in the refractory group and the initial treatment group was higher than that in the control group,but there was no significant difference in the expression of ST2 between the refractory group and the initial treatment group.(3)At the level of mRNA expression,the levels of IL-31 and IL-33 in the refractory group and the initial treatment group were higher than those in the control group.Furthermore,the levels of IL-31 and IL-33 mRNA in the refractory group were higher than those in the initial treatment group.The ST2 mRNA levels in the refractory group and the initial treatment group were higher than that in the control group,but there was no significant difference between the refractory group and the initial treatmentgroup.(4)There was no significant difference between the three groups in the total IgE levels in the peripheral blood.Conclusion:(1)a large number of eosinophil infiltration in the eosinophils of patients with refractory sinusitis and common chronic sinusitis,which is an important pathological manifestation,may also play an important role in the development of RRS.(2)interleukinIL-31 and interleukinIL-33 play animportant role in the occurrence and development of refractory sinusitis.Inhibition of IL-31 and IL-33 expression may provide new ideas for the treatment of refractory sinusitis.