Effects Of High Sugar Diet On The Proliferation And Differentiation Of Intestinal Stem Cells In Drosophila Melanogaster

Sugar is the main source of energy in all organisms to sustain basic activities required for life.With the economy’s development and the improvement of living condition,people’s eating habits have been changed significantly.Many people inclined to eat foods containing high concentration of sugar,while such eating habits bring us many diseases,such as obesity,heart disease and insulin resistance.Gut is the longest digestive tract in human being,and it is important for digestion and absorption in organisms.Meanwhile,the intestinal mucosa is the first line of defense against pathogen,playing an important role in immune response.To study the effect of high sugar diet on the gut immunity,we chose model organism Drosophila melanogaster as experimental material,since it shows many similarities to mammals in gene function and metabolic patterns.The concentration of sucrose in normal culture medium is 0.15M and 1M in the experiment.Put eggs in high sugar medium and make them grow up in such condition.After eclosion,we detected the number and shape of intestinal epithelium cells,signal pathway factors,the level of reactive oxygen species,etc.The results showed that high sugar diet promotes intestinal epithelium cells differentiation and the level of reactive oxygen species,with activating JNK pathway and increasing the number of dead cells.Moreover,we found that the variety and content of symbiotic bacteria changes significantly in the Drosophila guts which fed with high sugar medium.This article states the effect of high sugar diet on the intestinal immune of Drosophila,and provides theory evidence in studying the effect of high sugar diet on the organism and diseases related with the gut metabolic.