| Objectives:To observe the primary culture model of the mandibular condylar chondrocytes(MCCs)in SD rat under 280mT static magnetic field,the changing expression of type ? collagen,Indian Hedgehog,type ? collagen and the important regulatory factors Sox9 and Runx2 reflects the effect of static magnetic field on the maturation and differentiation of condylar cartilage.It is a theoretical basis for the further study of the effect of static magnetic field on the growth and development of maxillofacial cartilage and the clinical application of magnetic biological effect.Methods:The mandibular condylar cartilage was cultured in 2-3 days of SD newborn rats,and the primary condylar chondrocytes were cultivated,purified and cultured.Then the chondrocytes were divided into negative group(MCC)and static magnetic field group(SMC)which loaded 280mT static magnetic field.MCCs were identified by type ? collagen immunohistochemical staining and Toluidine Blue staining.The ultrastructure of the surface of Condylar chondrocytes was observed in the 3rd,8th,15th day by scanning electron microscope.The effect of static magnetic field on the expression of type ? collagen,IHH and type X collagen in the 3rd,8th,15th day of condylar chondrocytes was detected by RT-qPCR,and the protein expression of Sox9,Runx2 in the 3rd,8th,15th day after loading static magnetic field was detected by Western Blot method.Results:1.Type ? collagen immunohistochemical staining and toluidine blue staining were positive in the negative and magnetic field groups.2.Under the scanning electron microscope,with the increasing culture time,the surface of the condylar chondrocytes in the magnetic field group increased in the form of granular,scar-like,fibrous cord-like structure and intercellular synapses.3.Two groups of chondrocytes cultured from 3rd to 8th day,the cell matrix of type ? collagen secretion more,and IHH,type X collagen synthesis less,Sox9 highly expressed;from 8th to 15th day,IHH and X type of collagen secreted more,?-type collagen synthesis decreased,Runx2 highly expressed.After loading 280mT static magnetic field,the expression trend of type ? collagen,IHH,type ?collagen and regulation factor Sox9 and Runx2 was consistent with the negative group,and the expression of the magnetic field group is higher than that in the negative group at the same time point,and the difference is significant.Conclusions:1.Under the static magnetic field,the ultrastructure of the surface of the condylar chondrocytes had a certain change.And it suggested the structure and changes within the chondrocytes.It is indicated that the static magnetic field can cause the internal structure change of condylar chondrocytes.2.From the 3rd to 8th day,when condylar chondrocytes synthesized type ?collagen mainly,cells were in the early stage of maturation and differentiation,and static magnetic field mainly promoted ? collagen,Sox9 expression.From the 8th to 15th day,with secretion of IHH and X type of collagen,condylar chondrocytes were in the stage of hypertrophy,and static magnetic field promoted the expression of IHH,type X collagen and Runx2.3.Static magnetic field can promote the secretion of the matrix of condylar chondrocytes and the differentiation of condylar chondrocytes. |
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